Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes

Laïla Aïcha Hanafi; Marilène Bolduc; Marie Ève Laliberté Gagné; Florent Dufour; Yves Langelier; Mohammed Rachid Boulassel; Jean Pierre Routy; Denis Leclerc; Réjean Lapointe

doi:10.1016/j.vaccine.2010.06.024

Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes

Laïla Aïcha Hanafi, Marilène Bolduc, Marie Ève Laliberté Gagné, Florent Dufour, Yves Langelier, Mohammed Rachid Boulassel, Jean Pierre Routy, Denis Leclerc, Réjean Lapointe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.

Original language	English
Pages (from-to)	5617-5626
Number of pages	10
Journal	Vaccine
Volume	28
Issue number	34
DOIs	https://doi.org/10.1016/j.vaccine.2010.06.024
Publication status	Published - Aug 2010
Externally published	Yes

Keywords

Cross-presentation
Potexvirus
Virus-like particles

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2010.06.024

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title = "Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes",

abstract = "Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.",

keywords = "Cross-presentation, Potexvirus, Virus-like particles",

author = "Hanafi, {La{\"i}la A{\"i}cha} and Maril{\`e}ne Bolduc and Gagn{\'e}, {Marie {\`E}ve Lalibert{\'e}} and Florent Dufour and Yves Langelier and Boulassel, {Mohammed Rachid} and Routy, {Jean Pierre} and Denis Leclerc and R{\'e}jean Lapointe",

note = "Funding Information: This work was supported by a grant from the Cancer Research Society (CRS) and the Canadian Institutes of Health Research (CIHR). Financial support: R.L. and J.P.R. are recipients of Fonds de la recherche en sant{\'e} du Qu{\'e}bec (FRSQ) scholarships. We thank Ovid Da Silva from the “Bureau d{\textquoteright}aide {\`a} la recherche” (From Centre Hospitalier de l{\textquoteright}Universit{\'e} de Montr{\'e}al) for text editing. ",

year = "2010",

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doi = "10.1016/j.vaccine.2010.06.024",

language = "English",

volume = "28",

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AU - Hanafi, Laïla Aïcha

AU - Bolduc, Marilène

AU - Gagné, Marie Ève Laliberté

AU - Dufour, Florent

AU - Langelier, Yves

AU - Boulassel, Mohammed Rachid

AU - Routy, Jean Pierre

AU - Leclerc, Denis

AU - Lapointe, Réjean

N1 - Funding Information: This work was supported by a grant from the Cancer Research Society (CRS) and the Canadian Institutes of Health Research (CIHR). Financial support: R.L. and J.P.R. are recipients of Fonds de la recherche en santé du Québec (FRSQ) scholarships. We thank Ovid Da Silva from the “Bureau d’aide à la recherche” (From Centre Hospitalier de l’Université de Montréal) for text editing.

PY - 2010/8

Y1 - 2010/8

N2 - Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.

AB - Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.

KW - Cross-presentation

KW - Potexvirus

KW - Virus-like particles

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Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes

Abstract

Keywords

ASJC Scopus subject areas

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