Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes

Laïla Aïcha Hanafi, Marilène Bolduc, Marie Ève Laliberté Gagné, Florent Dufour, Yves Langelier, Mohammed Rachid Boulassel, Jean Pierre Routy, Denis Leclerc, Réjean Lapointe

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13 Citations (Scopus)

Abstract

Chimeric VLPs made of papaya mosaic virus (PapMV) trigger a CTL response through antigenic presentation of epitopes on MHC class I. Here, a chimeric VLP composed of malva mosaic virus (MaMV) was shown to share similar properties. We demonstrated the capacity of both VLPs to enter human APCs. The chimeric constructions were cross-presented in CD40-activated B lymphocytes leading to in vitro expansion of antigen-specific T lymphocytes. We showed that high concentrations of chimeric MaMV induced cell death, suggesting that some modifications can trigger collateral effects in vitro. Results suggest that potexvirus VLPs are an attractive vaccine platform for inducing a CTL response.

Original languageEnglish
Pages (from-to)5617-5626
Number of pages10
JournalVaccine
Volume28
Issue number34
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Cross-presentation
  • Potexvirus
  • Virus-like particles

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Hanafi, L. A., Bolduc, M., Gagné, M. È. L., Dufour, F., Langelier, Y., Boulassel, M. R., Routy, J. P., Leclerc, D., & Lapointe, R. (2010). Two distinct chimeric potexviruses share antigenic cross-presentation properties of MHC class I epitopes. Vaccine, 28(34), 5617-5626. https://doi.org/10.1016/j.vaccine.2010.06.024