The effect of quorum-sensing blockers on the formation of marine microbial communities and larval attachment

Sergey Dobretsov, Hans Uwe Dahms, Huang Yili, Martin Wahl, Pei Yuan Qian

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We studied the effect of the quorum-sensing (QS) blockers 5-hydroxy-3[(1R)-1-hydroxypropyl]-4-methylfuran-2(5H)-one (FUR1), (5R)-3,4-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]furan-2(5H)-one (FUR2) and triclosan (TRI) on the formation of bacterial biofilms, and the effect of these biofilms on the larval attachment of the polychaete Hydroides elegans and the bryozoan Bugula neritina. 14-day-old subtidal biofilms were harvested from artificial substrata and were allowed to develop in the laboratory with and without QS blockers. QS blockers inhibited the production of violacein by the QS reporter strain Chromobacterium violaceum CV026 and did not affect the metabolic activity of bacteria in multispecies biofilms. At a concentration of 10-3 M all three tested compounds inhibited the establishment of microbial communities, but at one of 10-4 M only FUR2 inhibited establishment. The tested QS blockers caused changes in bacterial density and bacterial community structure, as revealed by terminal restriction fragment length polymorphism and FISH. The groups most affected by QS blockers were Alphaproteobacteria, Gammaproteobacteria and the Cytophagales. Larvae of H. elegans and B. neritina avoided settling on biofilms that had developed in the presence of QS blockers. Our results suggest that QS blockers directly control the formation of multi-species biofilms, and indirectly - by means of biofilm properties - affect larval attachment on these modified biofilms.

Original languageEnglish
Pages (from-to)177-188
Number of pages12
JournalFEMS Microbiology Ecology
Volume60
Issue number2
DOIs
Publication statusPublished - May 2007

Fingerprint

Quorum Sensing
Biofilms
biofilm
microbial community
Chromobacterium
Triclosan
Bacteroidetes
Alphaproteobacteria
Gammaproteobacteria
Bacterial Structures
effect
furan
bryozoan
polychaete
Restriction Fragment Length Polymorphisms
Larva
polymorphism
community structure
larva
Bacteria

Keywords

  • Antifouling
  • Furanones
  • Larval attachment
  • Microbial communities
  • Quorum-sensing inhibitors

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The effect of quorum-sensing blockers on the formation of marine microbial communities and larval attachment. / Dobretsov, Sergey; Dahms, Hans Uwe; Yili, Huang; Wahl, Martin; Qian, Pei Yuan.

In: FEMS Microbiology Ecology, Vol. 60, No. 2, 05.2007, p. 177-188.

Research output: Contribution to journalArticle

Dobretsov, Sergey ; Dahms, Hans Uwe ; Yili, Huang ; Wahl, Martin ; Qian, Pei Yuan. / The effect of quorum-sensing blockers on the formation of marine microbial communities and larval attachment. In: FEMS Microbiology Ecology. 2007 ; Vol. 60, No. 2. pp. 177-188.
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