The effect of quorum-sensing blockers on the formation of marine microbial communities and larval attachment

Sergey Dobretsov*, Hans Uwe Dahms, Huang Yili, Martin Wahl, Pei Yuan Qian

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

67 اقتباسات (Scopus)

ملخص

We studied the effect of the quorum-sensing (QS) blockers 5-hydroxy-3[(1R)-1-hydroxypropyl]-4-methylfuran-2(5H)-one (FUR1), (5R)-3,4-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]furan-2(5H)-one (FUR2) and triclosan (TRI) on the formation of bacterial biofilms, and the effect of these biofilms on the larval attachment of the polychaete Hydroides elegans and the bryozoan Bugula neritina. 14-day-old subtidal biofilms were harvested from artificial substrata and were allowed to develop in the laboratory with and without QS blockers. QS blockers inhibited the production of violacein by the QS reporter strain Chromobacterium violaceum CV026 and did not affect the metabolic activity of bacteria in multispecies biofilms. At a concentration of 10-3 M all three tested compounds inhibited the establishment of microbial communities, but at one of 10-4 M only FUR2 inhibited establishment. The tested QS blockers caused changes in bacterial density and bacterial community structure, as revealed by terminal restriction fragment length polymorphism and FISH. The groups most affected by QS blockers were Alphaproteobacteria, Gammaproteobacteria and the Cytophagales. Larvae of H. elegans and B. neritina avoided settling on biofilms that had developed in the presence of QS blockers. Our results suggest that QS blockers directly control the formation of multi-species biofilms, and indirectly - by means of biofilm properties - affect larval attachment on these modified biofilms.

اللغة الأصليةEnglish
الصفحات (من إلى)177-188
عدد الصفحات12
دوريةFEMS Microbiology Ecology
مستوى الصوت60
رقم الإصدار2
المعرِّفات الرقمية للأشياء
حالة النشرPublished - مايو 2007
منشور خارجيًانعم

ASJC Scopus subject areas

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