Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51

Sanket Joshi; Sanjay Yadav; Anuradha Nerurkar; Anjana J. Desai

Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51

Sanket Joshi, Sanjay Yadav, Anuradha Nerurkar, Anjana J. Desai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

46 Citations (Scopus)

Abstract

The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H₃PO₄, CaCl₂, H₃BO₃, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO₃, 4.4; MgSO₄.7H₂O, 0.8; KCl, 0.4; CaCl₂, 0.27; H₃PO₄, 1.0 ml/l; and trace elements (mg/l): H₃BO₃, 0.25; CuSO₄, 0.6; MnSO₄, 2.2; Na₂MoO₄, 0.5; ZnSO₄, 6.0; FeSO₄, 8.0; CoCl₂, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.

Original language	English
Pages (from-to)	313-319
Number of pages	7
Journal	Journal of Microbiology and Biotechnology
Volume	17
Issue number	2
Publication status	Published - Feb 2007
Externally published	Yes

Keywords

Bacillus licheniformis
Biosurfactant
Box-Behnken
Critical micelle dilution (CMD)
Plackett-burman

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Cite this

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title = "Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51",

abstract = "The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4.7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.",

keywords = "Bacillus licheniformis, Biosurfactant, Box-Behnken, Critical micelle dilution (CMD), Plackett-burman",

author = "Sanket Joshi and Sanjay Yadav and Anuradha Nerurkar and Desai, {Anjana J.}",

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AU - Joshi, Sanket

AU - Yadav, Sanjay

AU - Nerurkar, Anuradha

AU - Desai, Anjana J.

PY - 2007/2

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N2 - The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4.7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.

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Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51

Abstract

Keywords

ASJC Scopus subject areas

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