Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51

Sanket Joshi, Sanjay Yadav, Anuradha Nerurkar, Anjana J. Desai

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37 Citations (Scopus)


The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4.7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalJournal of Microbiology and Biotechnology
Issue number2
Publication statusPublished - Feb 2007



  • Bacillus licheniformis
  • Biosurfactant
  • Box-Behnken
  • Critical micelle dilution (CMD)
  • Plackett-burman

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

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