Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51

Sanket Joshi, Sanjay Yadav, Anuradha Nerurkar, Anjana J. Desai

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4.7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number2
Publication statusPublished - Feb 2007

Fingerprint

Bacilli
Edetic Acid
Ethylenediaminetetraacetic acid
Nutritional Requirements
Trace Elements
Micelles
Trace elements
Glucose
Dilution
Screening
Bacillus licheniformis
sodium molybdate(VI)

Keywords

  • Bacillus licheniformis
  • Biosurfactant
  • Box-Behnken
  • Critical micelle dilution (CMD)
  • Plackett-burman

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51. / Joshi, Sanket; Yadav, Sanjay; Nerurkar, Anuradha; Desai, Anjana J.

In: Journal of Microbiology and Biotechnology, Vol. 17, No. 2, 02.2007, p. 313-319.

Research output: Contribution to journalArticle

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