Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51

Sanket Joshi, Sanjay Yadav, Anuradha Nerurkar, Anjana J. Desai*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

43 اقتباسات (Scopus)

ملخص

The nutritional medium requirement for biosurfactant production by Bacillus licheniformis K51 was optimized. The important medium components, identified by the initial screening method of Plackett-Burman, were H3PO4, CaCl2, H3BO3, and Na-EDTA. Box-Behnken response surface methodology was applied to further optimize biosurfactant production. The optimal concentrations for higher production of biosurfactants were (g/l): glucose, 1.1; NaNO3, 4.4; MgSO4.7H2O, 0.8; KCl, 0.4; CaCl2, 0.27; H3PO4, 1.0 ml/l; and trace elements (mg/l): H3BO3, 0.25; CuSO4, 0.6; MnSO4, 2.2; Na2MoO4, 0.5; ZnSO4, 6.0; FeSO4, 8.0; CoCl2, 1.0; and Na-EDTA, 30.0. Using this statistical optimization method, the relative biosurfactant yield as critical micelle dilution (CMD) was increased from 10× to 105×, which is ten times higher than the non-optimized rich medium.

اللغة الأصليةEnglish
الصفحات (من إلى)313-319
عدد الصفحات7
دوريةJournal of Microbiology and Biotechnology
مستوى الصوت17
رقم الإصدار2
حالة النشرPublished - فبراير 2007
منشور خارجيًانعم

ASJC Scopus subject areas

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