Production of efrapeptins by Tolypocladium species and evaluation of their insecticidal and antimicrobial properties

A. R. Bandani, B. P S Khambay, J. L. Faull, R. Newton, M. Deadman, T. M. Butt

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

This study shows for the first time that Tolypocladium species produce efrapeptins, a group of toxic peptides, in vivo but the quantities are too small to account for insect death, suggesting that these insecticidal compounds work in concert with other pathogenicity determinants. There is inter- and intraspecific variation in efrapeptin production in vitro by Tolypocladium species. T. parasiticum produced only efrapeptin E, in small quantities. Efrapeptins were detectable 48 h after inoculation and increased with biomass. The relative amounts of individual efrapeptins (C, D, E, F, G) produced by T. niveum in vitro were D > E > F > C > G but in vivo they were D > F > C > E > G. Efrapeptins were toxic to a wide range of insects when injected into the haemocoel. Mortality was dose-related. Efrapeptins also exhibited limited antifungal and antibacterial activity. Micrococcus luteus was considered an excellent indicator of efrapeptin presence in culture filtrate extracts because of its extreme sensitivity to these compounds.

Original languageEnglish
Pages (from-to)537-544
Number of pages8
JournalMycological Research
Volume104
Issue number5
DOIs
Publication statusPublished - May 2000

Fingerprint

efrapeptin
Tolypocladium
insecticidal properties
anti-infective properties
insect
hemocoel
Micrococcus luteus
insects
interspecific variation
intraspecific variation
culture filtrates
pathogenicity
peptide
inoculation
Poisons
peptides
death
mortality
Insects
biomass

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Production of efrapeptins by Tolypocladium species and evaluation of their insecticidal and antimicrobial properties. / Bandani, A. R.; Khambay, B. P S; Faull, J. L.; Newton, R.; Deadman, M.; Butt, T. M.

In: Mycological Research, Vol. 104, No. 5, 05.2000, p. 537-544.

Research output: Contribution to journalArticle

Bandani, A. R. ; Khambay, B. P S ; Faull, J. L. ; Newton, R. ; Deadman, M. ; Butt, T. M. / Production of efrapeptins by Tolypocladium species and evaluation of their insecticidal and antimicrobial properties. In: Mycological Research. 2000 ; Vol. 104, No. 5. pp. 537-544.
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