Abstract
This study shows for the first time that Tolypocladium species produce efrapeptins, a group of toxic peptides, in vivo but the quantities are too small to account for insect death, suggesting that these insecticidal compounds work in concert with other pathogenicity determinants. There is inter- and intraspecific variation in efrapeptin production in vitro by Tolypocladium species. T. parasiticum produced only efrapeptin E, in small quantities. Efrapeptins were detectable 48 h after inoculation and increased with biomass. The relative amounts of individual efrapeptins (C, D, E, F, G) produced by T. niveum in vitro were D > E > F > C > G but in vivo they were D > F > C > E > G. Efrapeptins were toxic to a wide range of insects when injected into the haemocoel. Mortality was dose-related. Efrapeptins also exhibited limited antifungal and antibacterial activity. Micrococcus luteus was considered an excellent indicator of efrapeptin presence in culture filtrate extracts because of its extreme sensitivity to these compounds.
| Original language | English |
|---|---|
| Pages (from-to) | 537-544 |
| Number of pages | 8 |
| Journal | Mycological Research |
| Volume | 104 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2000 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Plant Science
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Production of efrapeptins by Tolypocladium species and evaluation of their insecticidal and antimicrobial properties. / Bandani, A. R.; Khambay, B. P S; Faull, J. L.; Newton, R.; Deadman, M.; Butt, T. M.
In: Mycological Research, Vol. 104, No. 5, 05.2000, p. 537-544.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Production of efrapeptins by Tolypocladium species and evaluation of their insecticidal and antimicrobial properties
AU - Bandani, A. R.
AU - Khambay, B. P S
AU - Faull, J. L.
AU - Newton, R.
AU - Deadman, M.
AU - Butt, T. M.
PY - 2000/5
Y1 - 2000/5
N2 - This study shows for the first time that Tolypocladium species produce efrapeptins, a group of toxic peptides, in vivo but the quantities are too small to account for insect death, suggesting that these insecticidal compounds work in concert with other pathogenicity determinants. There is inter- and intraspecific variation in efrapeptin production in vitro by Tolypocladium species. T. parasiticum produced only efrapeptin E, in small quantities. Efrapeptins were detectable 48 h after inoculation and increased with biomass. The relative amounts of individual efrapeptins (C, D, E, F, G) produced by T. niveum in vitro were D > E > F > C > G but in vivo they were D > F > C > E > G. Efrapeptins were toxic to a wide range of insects when injected into the haemocoel. Mortality was dose-related. Efrapeptins also exhibited limited antifungal and antibacterial activity. Micrococcus luteus was considered an excellent indicator of efrapeptin presence in culture filtrate extracts because of its extreme sensitivity to these compounds.
AB - This study shows for the first time that Tolypocladium species produce efrapeptins, a group of toxic peptides, in vivo but the quantities are too small to account for insect death, suggesting that these insecticidal compounds work in concert with other pathogenicity determinants. There is inter- and intraspecific variation in efrapeptin production in vitro by Tolypocladium species. T. parasiticum produced only efrapeptin E, in small quantities. Efrapeptins were detectable 48 h after inoculation and increased with biomass. The relative amounts of individual efrapeptins (C, D, E, F, G) produced by T. niveum in vitro were D > E > F > C > G but in vivo they were D > F > C > E > G. Efrapeptins were toxic to a wide range of insects when injected into the haemocoel. Mortality was dose-related. Efrapeptins also exhibited limited antifungal and antibacterial activity. Micrococcus luteus was considered an excellent indicator of efrapeptin presence in culture filtrate extracts because of its extreme sensitivity to these compounds.
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U2 - 10.1017/S0953756299001859
DO - 10.1017/S0953756299001859
M3 - Article
VL - 104
SP - 537
EP - 544
JO - Fungal Biology
JF - Fungal Biology
SN - 1878-6146
IS - 5
ER -