Investigating the interactions of hyaluronan derivatives with biomolecules. The use of diffusional NMR techniques

Anna Taglienti, Francesco Cellesi, Vittorio Crescenzi, Paolo Sequi, Massimiliano Valentini, Nicola Tirelli

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The interactions between a biomaterial and biomolecules present in body fluids often determine the fate of the biomaterial. This paper presents a study on hyaluronan (HA)-containing materials (in soluble or colloidal form) that focuses on their interactions with lipids and proteins and for the first time uses PFG NMR as an analytical technique for probing these events. The interactions of HA-based polymers with phospholipids (DPPC and DPPG liposomes) are shown to depend both on charge and hydrophobicity factors. Despite the difference in behavior between albumin (substantially non-adhesive) and fibrinogen (adhesive), the interactions of the polymers with proteins do not seem to be based on hydrophobic effects but on surface polar interactions.

Original languageEnglish
Pages (from-to)611-622
Number of pages12
JournalMacromolecular Bioscience
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 7 2006

Keywords

  • "Stealth" character
  • Colloidal carriers
  • Hyaluronan
  • PFG NMR
  • Protein adsorption

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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