Immune responses to third stage larvae of Onchocerca volvulus in interferon-gamma and interleukin-4 knockout mice

Eugene H. Johnson, Sylvia Schynder-Candrian, Thiruchandurai V. Rajan, F. Kenneth Nelson, Sara Lustigman, David Abraham

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

To shed clarity on the dichotomy of reported results relative to the significance of T helper-1 vs T helper-2 immune responses in onchocerciasis, we compared the survivability of Onchocerca volvulus third-stage larvae (L3) in immunized mice that had either a targeted disruption of the Interleukin-4 or Interferon-gamma gene. Treatment groups consisted of control mice and mice immunized with irradiated O. volvulus L3. All mice were challenged with diffusion chambers containing viable L3. Vaccinated IL-4(-/-) were unable to kill this larval target. In contrast, vaccinated IFN-γ(-/-) and C57BL/6 mice, exhibited high levels of killing, had elevated levels of IL-4 and significantly greater numbers of eosinophils in their diffusion chambers than the IL-4(-/-). Whereas, levels of IFN-γ in all three groups of immunized mice were equivalent to those of control mice, levels of IL-5 were elevated, even in the IL-4(-/-), indicating that cytokines other than IL-4 were involved in its production. The protective immune response to third-stage larvae of O. volvulus in mice vaccinated with irradiated larvae has an absolute IL-4 requirement.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalParasite Immunology
Volume20
Issue number7
DOIs
Publication statusPublished - 1998

Fingerprint

Onchocerca volvulus
Knockout Mice
Interleukin-4
Interferon-gamma
Larva
Onchocerciasis
Interleukin-5
Inbred C57BL Mouse
Eosinophils
Cytokines
Control Groups

Keywords

  • Immunity
  • Infective larvae
  • Interferon-gamma knockouts
  • Interleukin-4 knockouts
  • O. volvulus

ASJC Scopus subject areas

  • Immunology
  • Parasitology

Cite this

Immune responses to third stage larvae of Onchocerca volvulus in interferon-gamma and interleukin-4 knockout mice. / Johnson, Eugene H.; Schynder-Candrian, Sylvia; Rajan, Thiruchandurai V.; Nelson, F. Kenneth; Lustigman, Sara; Abraham, David.

In: Parasite Immunology, Vol. 20, No. 7, 1998, p. 319-324.

Research output: Contribution to journalArticle

Johnson, Eugene H. ; Schynder-Candrian, Sylvia ; Rajan, Thiruchandurai V. ; Nelson, F. Kenneth ; Lustigman, Sara ; Abraham, David. / Immune responses to third stage larvae of Onchocerca volvulus in interferon-gamma and interleukin-4 knockout mice. In: Parasite Immunology. 1998 ; Vol. 20, No. 7. pp. 319-324.
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