Immune responses to third stage larvae of Onchocerca volvulus in interferon-gamma and interleukin-4 knockout mice

Eugene H. Johnson*, Sylvia Schynder-Candrian, Thiruchandurai V. Rajan, F. Kenneth Nelson, Sara Lustigman, David Abraham

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء

34 اقتباسات (Scopus)

ملخص

To shed clarity on the dichotomy of reported results relative to the significance of T helper-1 vs T helper-2 immune responses in onchocerciasis, we compared the survivability of Onchocerca volvulus third-stage larvae (L3) in immunized mice that had either a targeted disruption of the Interleukin-4 or Interferon-gamma gene. Treatment groups consisted of control mice and mice immunized with irradiated O. volvulus L3. All mice were challenged with diffusion chambers containing viable L3. Vaccinated IL-4(-/-) were unable to kill this larval target. In contrast, vaccinated IFN-γ(-/-) and C57BL/6 mice, exhibited high levels of killing, had elevated levels of IL-4 and significantly greater numbers of eosinophils in their diffusion chambers than the IL-4(-/-). Whereas, levels of IFN-γ in all three groups of immunized mice were equivalent to those of control mice, levels of IL-5 were elevated, even in the IL-4(-/-), indicating that cytokines other than IL-4 were involved in its production. The protective immune response to third-stage larvae of O. volvulus in mice vaccinated with irradiated larvae has an absolute IL-4 requirement.

اللغة الأصليةEnglish
الصفحات (من إلى)319-324
عدد الصفحات6
دوريةParasite Immunology
مستوى الصوت20
رقم الإصدار7
المعرِّفات الرقمية للأشياء
حالة النشرPublished - 1998

ASJC Scopus subject areas

  • ???subjectarea.asjc.2400.2405???
  • ???subjectarea.asjc.2400.2403???

بصمة

أدرس بدقة موضوعات البحث “Immune responses to third stage larvae of Onchocerca volvulus in interferon-gamma and interleukin-4 knockout mice'. فهما يشكلان معًا بصمة فريدة.

قم بذكر هذا