Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)

Jørgen Stenvik; Mette Serine Wesmajervi; Kjersti Turid Fjalestad; Børge Damsgård; Madjid Delghandi

doi:10.1111/j.1471-8286.2006.01450.x

Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)

Jørgen Stenvik, Mette Serine Wesmajervi, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.

Original language	English
Pages (from-to)	1105-1107
Number of pages	3
Journal	Molecular Ecology Notes
Volume	6
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01450.x
Publication status	Published - Dec 2006
Externally published	Yes

Keywords

Atlantic cod
Expressed sequence tags (ESTs)
Gadus morhua
Microsatellites
Type I markers
cDNA

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry,Genetics and Molecular Biology

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10.1111/j.1471-8286.2006.01450.x

Cite this

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abstract = "Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.",

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AU - Stenvik, Jørgen

AU - Wesmajervi, Mette Serine

AU - Fjalestad, Kjersti Turid

AU - Damsgård, Børge

AU - Delghandi, Madjid

PY - 2006/12

Y1 - 2006/12

N2 - Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.

AB - Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.

KW - Atlantic cod

KW - Expressed sequence tags (ESTs)

KW - Gadus morhua

KW - Microsatellites

KW - Type I markers

KW - cDNA

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Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)

Abstract

Keywords

ASJC Scopus subject areas

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