Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)

Jørgen Stenvik, Mette Serine Wesmajervi, Kjersti Turid Fjalestad, Børge Damsgård, Madjid Delghandi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.

Original languageEnglish
Pages (from-to)1105-1107
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number4
DOIs
Publication statusPublished - Dec 2006

Keywords

  • Atlantic cod
  • cDNA
  • Expressed sequence tags (ESTs)
  • Gadus morhua
  • Microsatellites
  • Type I markers

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

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