TY - JOUR
T1 - Development of 25 gene-associated microsatellite markers of Atlantic cod (Gadus morhua L.)
AU - Stenvik, Jørgen
AU - Wesmajervi, Mette Serine
AU - Fjalestad, Kjersti Turid
AU - Damsgård, Børge
AU - Delghandi, Madjid
PY - 2006/12
Y1 - 2006/12
N2 - Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.
AB - Microsatellites were identified by screening 2294 GenBank entries available for Atlantic cod (Gadus morhua L.), mainly representing expressed sequence tags and cDNA sequences. Ninety-two novel microsatellite loci (tetra-, tri- and dinucleotides) were characterized on 96 individuals. This strategy yielded 25 gene-associated polymorphic microsatellite markers (11 tri- and 14 dinucleotides) with two to 20 alleles and an average heterozygosity of 0.48 in the population studied (range 0.02-0.89). One marker exhibited significant homozygote excess, and one of the primer pairs amplified two linked markers. The gene identity was determined at nine of the loci, confirming the associated microsatellites as type I markers.
KW - Atlantic cod
KW - Expressed sequence tags (ESTs)
KW - Gadus morhua
KW - Microsatellites
KW - Type I markers
KW - cDNA
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U2 - 10.1111/j.1471-8286.2006.01450.x
DO - 10.1111/j.1471-8286.2006.01450.x
M3 - Article
AN - SCOPUS:33751307458
SN - 1471-8278
VL - 6
SP - 1105
EP - 1107
JO - Molecular Ecology Notes
JF - Molecular Ecology Notes
IS - 4
ER -