Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation

B. Poonkuzhali, J. Victoria, R. Selvakumar, D. Dennison, A. Srivastava, L. Jeyaseelan, M. Chandy, A. S. Kanagasabapathy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.

Original languageEnglish
Pages (from-to)39-41
Number of pages3
JournalIndian Journal of Medical Research
Volume102
Issue numberJULY
Publication statusPublished - 1995

Keywords

  • Cyclosporine A
  • Enzyme multiplied immunoassay technique
  • High performance liquid chromatography

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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