Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation

B. Poonkuzhali; J. Victoria; R. Selvakumar; D. Dennison; A. Srivastava; L. Jeyaseelan; M. Chandy; A. S. Kanagasabapathy

Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation

B. Poonkuzhali, J. Victoria, R. Selvakumar, D. Dennison, A. Srivastava, L. Jeyaseelan, M. Chandy, A. S. Kanagasabapathy^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.

Original language	English
Pages (from-to)	39-41
Number of pages	3
Journal	Indian Journal of Medical Research
Volume	102
Issue number	JULY
Publication status	Published - 1995
Externally published	Yes

Keywords

Cyclosporine A
Enzyme multiplied immunoassay technique
High performance liquid chromatography

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology

Cite this

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title = "Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation",

abstract = "Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.",

keywords = "Cyclosporine A, Enzyme multiplied immunoassay technique, High performance liquid chromatography",

author = "B. Poonkuzhali and J. Victoria and R. Selvakumar and D. Dennison and A. Srivastava and L. Jeyaseelan and M. Chandy and Kanagasabapathy, {A. S.}",

year = "1995",

language = "English",

volume = "102",

pages = "39--41",

journal = "Indian Journal of Medical Research",

issn = "0971-5916",

publisher = "Indian Council of Medical Research",

number = "JULY",

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TY - JOUR

T1 - Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation

AU - Poonkuzhali, B.

AU - Victoria, J.

AU - Selvakumar, R.

AU - Dennison, D.

AU - Srivastava, A.

AU - Jeyaseelan, L.

AU - Chandy, M.

AU - Kanagasabapathy, A. S.

PY - 1995

Y1 - 1995

N2 - Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.

AB - Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.

KW - Cyclosporine A

KW - Enzyme multiplied immunoassay technique

KW - High performance liquid chromatography

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M3 - Article

C2 - 7558209

AN - SCOPUS:0029088819

SN - 0971-5916

VL - 102

SP - 39

EP - 41

JO - Indian Journal of Medical Research

JF - Indian Journal of Medical Research

IS - JULY

ER -

Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation

Abstract

Keywords

ASJC Scopus subject areas

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