TY - JOUR
T1 - Comparison of HPLC and EMIT methods of cyclosporine assay in blood after bone marrow transplantation
AU - Poonkuzhali, B.
AU - Victoria, J.
AU - Selvakumar, R.
AU - Dennison, D.
AU - Srivastava, A.
AU - Jeyaseelan, L.
AU - Chandy, M.
AU - Kanagasabapathy, A. S.
PY - 1995
Y1 - 1995
N2 - Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.
AB - Cyclosporine (CsA) analysis in blood from patients who had undergone bone marrow transplantation for various haematological disorders was done both by high performance liquid chromatography (HPLC) and enzyme multiplied immunoassay technique (EMIT) and the results were compared. HPLC kit from Biorad Laboratories, USA, and EMIT kit from SYVA, UK, were used. The procedure for EMIT was slightly modified in-house to suit the Hitachi 704 discrete selective analyser. The CsA values obtained by these two methods correlated well within the therapeutic range (r value 0.96), HPLC method being most suitable outside the therapeutic range. Although HPLC is the ideal method for CsA, EMIT is quite suitable and can be adopted by any laboratory with an autoanalyser incorporating our modified procedure.
KW - Cyclosporine A
KW - Enzyme multiplied immunoassay technique
KW - High performance liquid chromatography
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M3 - Article
C2 - 7558209
AN - SCOPUS:0029088819
SN - 0971-5916
VL - 102
SP - 39
EP - 41
JO - Indian Journal of Medical Research
JF - Indian Journal of Medical Research
IS - JULY
ER -