Biosurfactant production by Bacillus subtilis B30 and its application in enhancing oil recovery

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Abstract

The fermentative production of biosurfactants by Bacillus subtilis strain B30 and the evaluation of biosurfactant based enhanced oil recovery using core-flood were investigated. Different carbon sources (glucose, sucrose, starch, date molasses, cane molasses) were tested to determine the optimal biosurfactant production. The isolate B30 produced a biosurfactant that could reduce the surface tension and interfacial tension to 26.63. ±. 0.45. mN/m and 3.79. ±. 0.27. mN/m, respectively in less than 12. h in both glucose or date molasses based media. A crude biosurfactant concentration of 0.3-0.5. g/l and critical micelle dilution (CMD) values of 1:8 were observed. The biosurfactants gave stable emulsions with wide range of hydrocarbons including light and heavy crude oil. The biosurfactants were partially purified and identified as a mixture of lipopeptides similar to surfactin, using high performance thin layer chromatography and Fourier transform infrared spectroscopy. The biosurfactants were stable over wide range of pH, salinity and temperatures. The crude biosurfactant preparation enhanced light oil recovery by 17-26% and heavy oil recovery by 31% in core-flood studies. The results are indicative of the potential of the strain for the development of ex situ microbial enhanced oil recovery processes using glucose or date molasses based minimal media.

Original languageEnglish
Pages (from-to)324-333
Number of pages10
JournalColloids and Surfaces B: Biointerfaces
Volume114
DOIs
Publication statusPublished - Feb 1 2014

Fingerprint

Molasses
oil recovery
Bacillus
Bacilli
Bacillus subtilis
Oils
glucose
Glucose
Recovery
Surface Tension
Surface tension
interfacial tension
Crude oil
thin layer chromatography
Lipopeptides
Thin layer chromatography
Canes
starches
sucrose
Salinity

Keywords

  • Bacillus subtilis
  • Biosurfactant
  • Critical micelle dilution
  • Emulsification index
  • Microbial enhanced oil recovery
  • Surface and interfacial tension reduction

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

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title = "Biosurfactant production by Bacillus subtilis B30 and its application in enhancing oil recovery",
abstract = "The fermentative production of biosurfactants by Bacillus subtilis strain B30 and the evaluation of biosurfactant based enhanced oil recovery using core-flood were investigated. Different carbon sources (glucose, sucrose, starch, date molasses, cane molasses) were tested to determine the optimal biosurfactant production. The isolate B30 produced a biosurfactant that could reduce the surface tension and interfacial tension to 26.63. ±. 0.45. mN/m and 3.79. ±. 0.27. mN/m, respectively in less than 12. h in both glucose or date molasses based media. A crude biosurfactant concentration of 0.3-0.5. g/l and critical micelle dilution (CMD) values of 1:8 were observed. The biosurfactants gave stable emulsions with wide range of hydrocarbons including light and heavy crude oil. The biosurfactants were partially purified and identified as a mixture of lipopeptides similar to surfactin, using high performance thin layer chromatography and Fourier transform infrared spectroscopy. The biosurfactants were stable over wide range of pH, salinity and temperatures. The crude biosurfactant preparation enhanced light oil recovery by 17-26{\%} and heavy oil recovery by 31{\%} in core-flood studies. The results are indicative of the potential of the strain for the development of ex situ microbial enhanced oil recovery processes using glucose or date molasses based minimal media.",
keywords = "Bacillus subtilis, Biosurfactant, Critical micelle dilution, Emulsification index, Microbial enhanced oil recovery, Surface and interfacial tension reduction",
author = "Yahya Al-Wahaibi and Sanket Joshi and Saif Al-Bahry and Abdulkadir Elshafie and Ali Al-Bemani and Biji Shibulal",
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AU - Al-Wahaibi, Yahya

AU - Joshi, Sanket

AU - Al-Bahry, Saif

AU - Elshafie, Abdulkadir

AU - Al-Bemani, Ali

AU - Shibulal, Biji

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