TY - JOUR
T1 - Biosurfactant production by Bacillus subtilis B30 and its application in enhancing oil recovery
AU - Al-Wahaibi, Yahya
AU - Joshi, Sanket
AU - Al-Bahry, Saif
AU - Elshafie, Abdulkadir
AU - Al-Bemani, Ali
AU - Shibulal, Biji
N1 - Funding Information:
Authors acknowledge His Majesty Research Fund ( SR/SCI/BIOL/08/01 ) Sultan Qaboos University, Oman and the Petroleum Development of Oman ( CR/SCI/BIOL/07/02 ) for the research grants.
PY - 2014/2/1
Y1 - 2014/2/1
N2 - The fermentative production of biosurfactants by Bacillus subtilis strain B30 and the evaluation of biosurfactant based enhanced oil recovery using core-flood were investigated. Different carbon sources (glucose, sucrose, starch, date molasses, cane molasses) were tested to determine the optimal biosurfactant production. The isolate B30 produced a biosurfactant that could reduce the surface tension and interfacial tension to 26.63. ±. 0.45. mN/m and 3.79. ±. 0.27. mN/m, respectively in less than 12. h in both glucose or date molasses based media. A crude biosurfactant concentration of 0.3-0.5. g/l and critical micelle dilution (CMD) values of 1:8 were observed. The biosurfactants gave stable emulsions with wide range of hydrocarbons including light and heavy crude oil. The biosurfactants were partially purified and identified as a mixture of lipopeptides similar to surfactin, using high performance thin layer chromatography and Fourier transform infrared spectroscopy. The biosurfactants were stable over wide range of pH, salinity and temperatures. The crude biosurfactant preparation enhanced light oil recovery by 17-26% and heavy oil recovery by 31% in core-flood studies. The results are indicative of the potential of the strain for the development of ex situ microbial enhanced oil recovery processes using glucose or date molasses based minimal media.
AB - The fermentative production of biosurfactants by Bacillus subtilis strain B30 and the evaluation of biosurfactant based enhanced oil recovery using core-flood were investigated. Different carbon sources (glucose, sucrose, starch, date molasses, cane molasses) were tested to determine the optimal biosurfactant production. The isolate B30 produced a biosurfactant that could reduce the surface tension and interfacial tension to 26.63. ±. 0.45. mN/m and 3.79. ±. 0.27. mN/m, respectively in less than 12. h in both glucose or date molasses based media. A crude biosurfactant concentration of 0.3-0.5. g/l and critical micelle dilution (CMD) values of 1:8 were observed. The biosurfactants gave stable emulsions with wide range of hydrocarbons including light and heavy crude oil. The biosurfactants were partially purified and identified as a mixture of lipopeptides similar to surfactin, using high performance thin layer chromatography and Fourier transform infrared spectroscopy. The biosurfactants were stable over wide range of pH, salinity and temperatures. The crude biosurfactant preparation enhanced light oil recovery by 17-26% and heavy oil recovery by 31% in core-flood studies. The results are indicative of the potential of the strain for the development of ex situ microbial enhanced oil recovery processes using glucose or date molasses based minimal media.
KW - Bacillus subtilis
KW - Biosurfactant
KW - Critical micelle dilution
KW - Emulsification index
KW - Microbial enhanced oil recovery
KW - Surface and interfacial tension reduction
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U2 - 10.1016/j.colsurfb.2013.09.022
DO - 10.1016/j.colsurfb.2013.09.022
M3 - Article
C2 - 24240116
AN - SCOPUS:84887672888
SN - 0927-7765
VL - 114
SP - 324
EP - 333
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -