Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces

Yasmin El Tahir, Paavo Toivanen, Mikael Skurnik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe the application of an enzyme immunoassay (EIA) for detecting bacteria bound to a solid surface. Different Yersinia enterocolitica and Escherichia coli strains, expressing the YadA protein, type 1 or type P fimbriae were used as models for this study. The assay was used to detect bacteria bound to fixed tissues or to glass slides coated with extracellular matrix molecules (collagen, laminin or fibronectin). E. coli specific antiserum (B357, Dakopatts, Glostrup, Denmark) and peroxidase conjugated antiserum (P217) were used to detect all E. coli strains used in the study. The bacterial binding could be monitored with a linear detection range between 105 and 108 bacteria. Most importantly, dose dependent inhibition of bacterial binding by soluble extracellular matrix molecules could be measured.

Original languageEnglish
Pages (from-to)165-183
Number of pages19
JournalJournal of Immunoassay
Volume18
Issue number2
Publication statusPublished - 1997

Keywords

  • Bacterial adhesion
  • E. coil
  • Extracellular matrix molecules
  • Fimbriae
  • YadA
  • Yersinia

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Fingerprint Dive into the research topics of 'Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces'. Together they form a unique fingerprint.

  • Cite this