Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces

Yasmin El Tahir, Paavo Toivanen, Mikael Skurnik

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe the application of an enzyme immunoassay (EIA) for detecting bacteria bound to a solid surface. Different Yersinia enterocolitica and Escherichia coli strains, expressing the YadA protein, type 1 or type P fimbriae were used as models for this study. The assay was used to detect bacteria bound to fixed tissues or to glass slides coated with extracellular matrix molecules (collagen, laminin or fibronectin). E. coli specific antiserum (B357, Dakopatts, Glostrup, Denmark) and peroxidase conjugated antiserum (P217) were used to detect all E. coli strains used in the study. The bacterial binding could be monitored with a linear detection range between 105 and 108 bacteria. Most importantly, dose dependent inhibition of bacterial binding by soluble extracellular matrix molecules could be measured.

Original languageEnglish
Pages (from-to)165-183
Number of pages19
JournalJournal of Immunoassay
Volume18
Issue number2
Publication statusPublished - 1997

Fingerprint

Immunoenzyme Techniques
Escherichia coli
Bacteria
Extracellular Matrix
Immune Sera
Enzymes
Yersinia enterocolitica
Molecules
Laminin
Denmark
Fibronectins
Peroxidase
Glass
Assays
Collagen
Tissue
Proteins

Keywords

  • Bacterial adhesion
  • E. coil
  • Extracellular matrix molecules
  • Fimbriae
  • YadA
  • Yersinia

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces. / El Tahir, Yasmin; Toivanen, Paavo; Skurnik, Mikael.

In: Journal of Immunoassay, Vol. 18, No. 2, 1997, p. 165-183.

Research output: Contribution to journalArticle

@article{771ad465809f4cbda3fad08ab7176775,
title = "Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces",
abstract = "We describe the application of an enzyme immunoassay (EIA) for detecting bacteria bound to a solid surface. Different Yersinia enterocolitica and Escherichia coli strains, expressing the YadA protein, type 1 or type P fimbriae were used as models for this study. The assay was used to detect bacteria bound to fixed tissues or to glass slides coated with extracellular matrix molecules (collagen, laminin or fibronectin). E. coli specific antiserum (B357, Dakopatts, Glostrup, Denmark) and peroxidase conjugated antiserum (P217) were used to detect all E. coli strains used in the study. The bacterial binding could be monitored with a linear detection range between 105 and 108 bacteria. Most importantly, dose dependent inhibition of bacterial binding by soluble extracellular matrix molecules could be measured.",
keywords = "Bacterial adhesion, E. coil, Extracellular matrix molecules, Fimbriae, YadA, Yersinia",
author = "{El Tahir}, Yasmin and Paavo Toivanen and Mikael Skurnik",
year = "1997",
language = "English",
volume = "18",
pages = "165--183",
journal = "Journal of Immunoassay and Immunochemistry",
issn = "1532-1819",
publisher = "Taylor and Francis Ltd.",
number = "2",

}

TY - JOUR

T1 - Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces

AU - El Tahir, Yasmin

AU - Toivanen, Paavo

AU - Skurnik, Mikael

PY - 1997

Y1 - 1997

N2 - We describe the application of an enzyme immunoassay (EIA) for detecting bacteria bound to a solid surface. Different Yersinia enterocolitica and Escherichia coli strains, expressing the YadA protein, type 1 or type P fimbriae were used as models for this study. The assay was used to detect bacteria bound to fixed tissues or to glass slides coated with extracellular matrix molecules (collagen, laminin or fibronectin). E. coli specific antiserum (B357, Dakopatts, Glostrup, Denmark) and peroxidase conjugated antiserum (P217) were used to detect all E. coli strains used in the study. The bacterial binding could be monitored with a linear detection range between 105 and 108 bacteria. Most importantly, dose dependent inhibition of bacterial binding by soluble extracellular matrix molecules could be measured.

AB - We describe the application of an enzyme immunoassay (EIA) for detecting bacteria bound to a solid surface. Different Yersinia enterocolitica and Escherichia coli strains, expressing the YadA protein, type 1 or type P fimbriae were used as models for this study. The assay was used to detect bacteria bound to fixed tissues or to glass slides coated with extracellular matrix molecules (collagen, laminin or fibronectin). E. coli specific antiserum (B357, Dakopatts, Glostrup, Denmark) and peroxidase conjugated antiserum (P217) were used to detect all E. coli strains used in the study. The bacterial binding could be monitored with a linear detection range between 105 and 108 bacteria. Most importantly, dose dependent inhibition of bacterial binding by soluble extracellular matrix molecules could be measured.

KW - Bacterial adhesion

KW - E. coil

KW - Extracellular matrix molecules

KW - Fimbriae

KW - YadA

KW - Yersinia

UR - http://www.scopus.com/inward/record.url?scp=0030957397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030957397&partnerID=8YFLogxK

M3 - Article

C2 - 9134475

AN - SCOPUS:0030957397

VL - 18

SP - 165

EP - 183

JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

SN - 1532-1819

IS - 2

ER -