Application of an enzyme immunoassay to monitor bacterial binding and to measure inhibition of binding to different types of solid surfaces

Yasmin El Tahir*, Paavo Toivanen, Mikael Skurnik

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

2 اقتباسات (Scopus)

ملخص

We describe the application of an enzyme immunoassay (EIA) for detecting bacteria bound to a solid surface. Different Yersinia enterocolitica and Escherichia coli strains, expressing the YadA protein, type 1 or type P fimbriae were used as models for this study. The assay was used to detect bacteria bound to fixed tissues or to glass slides coated with extracellular matrix molecules (collagen, laminin or fibronectin). E. coli specific antiserum (B357, Dakopatts, Glostrup, Denmark) and peroxidase conjugated antiserum (P217) were used to detect all E. coli strains used in the study. The bacterial binding could be monitored with a linear detection range between 105 and 108 bacteria. Most importantly, dose dependent inhibition of bacterial binding by soluble extracellular matrix molecules could be measured.

اللغة الأصليةEnglish
الصفحات (من إلى)165-183
عدد الصفحات19
دوريةJournal of Immunoassay
مستوى الصوت18
رقم الإصدار2
المعرِّفات الرقمية للأشياء
حالة النشرPublished - 1997
منشور خارجيًانعم

ASJC Scopus subject areas

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