Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa

O. Habbal, S. S. Hasson, A. H. El-Hag, Z. Al-Mahrooqi, N. Al-Hashmi, Z. Al-Bimani, M. S. Al-Balushi, A. A. Al-Jabri

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Objective: To investigate the antibacterial activity of henna (Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms. Methods: Fresh henna samples were obtained from different regions of Oman as leaves and seeds. 100 g fresh and dry leaves and 50 g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days, respectively, with frequent agitation. The mixture was filtered, and the crude extract was collected. The crude extract was then heated, at 48 °C in a water bath to evaporate its liquid content. The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique. Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa (NCTC 10662) (P. aeruginosa) and eleven fresh clinical isolates of P. aeruginosa obtained from patients attending the Sultan Qaboos University Hospital (SQUH). 2-Hydroxy-p-Nathoqinone-Tech (2-HPNT, MW=174.16, C10H6O3) was included as control (at 50% concentration) along with the henna samples tested. Results: Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P. aeruginosa with henna samples obtained from Al-sharqyia region. Conclusions: Omani henna from Al-sharqyia region demonstrates high in vitro anti- P. aeruginosa activity compared with many henna samples from different regions of Oman.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalAsian Pacific Journal of Tropical Biomedicine
Volume1
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

Lawsonia Plant
Complex Mixtures
Pseudomonas aeruginosa
Seed
Oman
Ethanol
Anti-Bacterial Agents
Water
Liquids
Cotyledon
Baths
Seeds

Keywords

  • Antibacterial activity
  • Antibiotic susceptibility
  • Bacterial strain
  • Clinical isolate
  • Crude extract
  • Henna
  • Lawsonia inermis Linn
  • Micro-organism
  • Oman
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Medicine(all)

Cite this

Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa. / Habbal, O.; Hasson, S. S.; El-Hag, A. H.; Al-Mahrooqi, Z.; Al-Hashmi, N.; Al-Bimani, Z.; Al-Balushi, M. S.; Al-Jabri, A. A.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 1, No. 3, 2011, p. 173-176.

Research output: Contribution to journalArticle

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abstract = "Objective: To investigate the antibacterial activity of henna (Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms. Methods: Fresh henna samples were obtained from different regions of Oman as leaves and seeds. 100 g fresh and dry leaves and 50 g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days, respectively, with frequent agitation. The mixture was filtered, and the crude extract was collected. The crude extract was then heated, at 48 °C in a water bath to evaporate its liquid content. The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique. Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa (NCTC 10662) (P. aeruginosa) and eleven fresh clinical isolates of P. aeruginosa obtained from patients attending the Sultan Qaboos University Hospital (SQUH). 2-Hydroxy-p-Nathoqinone-Tech (2-HPNT, MW=174.16, C10H6O3) was included as control (at 50{\%} concentration) along with the henna samples tested. Results: Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P. aeruginosa with henna samples obtained from Al-sharqyia region. Conclusions: Omani henna from Al-sharqyia region demonstrates high in vitro anti- P. aeruginosa activity compared with many henna samples from different regions of Oman.",
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AU - Habbal, O.

AU - Hasson, S. S.

AU - El-Hag, A. H.

AU - Al-Mahrooqi, Z.

AU - Al-Hashmi, N.

AU - Al-Bimani, Z.

AU - Al-Balushi, M. S.

AU - Al-Jabri, A. A.

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N2 - Objective: To investigate the antibacterial activity of henna (Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms. Methods: Fresh henna samples were obtained from different regions of Oman as leaves and seeds. 100 g fresh and dry leaves and 50 g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days, respectively, with frequent agitation. The mixture was filtered, and the crude extract was collected. The crude extract was then heated, at 48 °C in a water bath to evaporate its liquid content. The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique. Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa (NCTC 10662) (P. aeruginosa) and eleven fresh clinical isolates of P. aeruginosa obtained from patients attending the Sultan Qaboos University Hospital (SQUH). 2-Hydroxy-p-Nathoqinone-Tech (2-HPNT, MW=174.16, C10H6O3) was included as control (at 50% concentration) along with the henna samples tested. Results: Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P. aeruginosa with henna samples obtained from Al-sharqyia region. Conclusions: Omani henna from Al-sharqyia region demonstrates high in vitro anti- P. aeruginosa activity compared with many henna samples from different regions of Oman.

AB - Objective: To investigate the antibacterial activity of henna (Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms. Methods: Fresh henna samples were obtained from different regions of Oman as leaves and seeds. 100 g fresh and dry leaves and 50 g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days, respectively, with frequent agitation. The mixture was filtered, and the crude extract was collected. The crude extract was then heated, at 48 °C in a water bath to evaporate its liquid content. The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique. Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa (NCTC 10662) (P. aeruginosa) and eleven fresh clinical isolates of P. aeruginosa obtained from patients attending the Sultan Qaboos University Hospital (SQUH). 2-Hydroxy-p-Nathoqinone-Tech (2-HPNT, MW=174.16, C10H6O3) was included as control (at 50% concentration) along with the henna samples tested. Results: Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P. aeruginosa with henna samples obtained from Al-sharqyia region. Conclusions: Omani henna from Al-sharqyia region demonstrates high in vitro anti- P. aeruginosa activity compared with many henna samples from different regions of Oman.

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