A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties

J. Michael Conlon, Agnes Sonnevend, Mahendra Patel, Vijayayasarathy Camasamudram, Norbert Nowotny, Erika Zilahi, Shawichi Iwamuro, Per F. Nielsen, Tibor Pál

Research output: Contribution to journal › Article

55 Citations (Scopus)

Abstract

Two peptides with antimicrobial and cytolytic properties were purified from an extract of the skin of Tago's brown frog Rana tagoi. The primary structure of one peptide (FLPILGKLLS10GIL·NH2) identifies it as a member of the temporin family, whereas the second peptide (AIGSILGALA10KGLPTLISWI20KNR·NH2) displays 78% sequence identity to melittin from the venom of the honeybee Apis florea. Compared with melittin, the melittin-related peptide (MRP) was equipotent in inhibiting the growth of the Gram-positive bacterium Staphylococcus aureus, 5-fold less potent against the Gram-negative bacterium Escherichia coli and against the fungal pathogen, Candida albicans. MRP was 13-fold less hemolytic than melittin against human erythrocytes and 4- and 5-fold less cytolytic against mouse EL4 T-lymphoma-derived cells and L929 fibroblasts, respectively. However, at non-cytotoxic concentrations (≤8μM), MRP did not protect HeLa cells from cell death produced by human rhinovirus type 2 infection.

Original languageEnglish
Pages (from-to)496-500
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number2
DOIs
Publication statusPublished - Jun 27 2003

Fingerprint

Melitten
Ranidae
Anura
Skin
Peptides
Bacteria
Bee Venoms
Rhinovirus
T-Cell Lymphoma
Candida
Venoms
Gram-Positive Bacteria
Pathogens
Cell death
Fibroblasts
Gram-Negative Bacteria
Candida albicans
HeLa Cells
Escherichia coli
Staphylococcus aureus

Keywords

  • Antimicrobial
  • Antiviral
  • Cytolytic
  • Frog skin
  • Hemolytic
  • Melittin
  • Temporin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties. / Conlon, J. Michael; Sonnevend, Agnes; Patel, Mahendra; Camasamudram, Vijayayasarathy; Nowotny, Norbert; Zilahi, Erika; Iwamuro, Shawichi; Nielsen, Per F.; Pál, Tibor.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 2, 27.06.2003, p. 496-500.

Research output: Contribution to journal › Article

Conlon, JM, Sonnevend, A, Patel, M, Camasamudram, V, Nowotny, N, Zilahi, E, Iwamuro, S, Nielsen, PF & Pál, T 2003, 'A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties', Biochemical and Biophysical Research Communications, vol. 306, no. 2, pp. 496-500. https://doi.org/10.1016/S0006-291X(03)00999-9
Conlon JM, Sonnevend A, Patel M, Camasamudram V, Nowotny N, Zilahi E et al. A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties. Biochemical and Biophysical Research Communications. 2003 Jun 27;306(2):496-500. https://doi.org/10.1016/S0006-291X(03)00999-9
Conlon, J. Michael ; Sonnevend, Agnes ; Patel, Mahendra ; Camasamudram, Vijayayasarathy ; Nowotny, Norbert ; Zilahi, Erika ; Iwamuro, Shawichi ; Nielsen, Per F. ; Pál, Tibor. / A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 2. pp. 496-500.
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