A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties

J. Michael Conlon; Agnes Sonnevend; Mahendra Patel; Vijayayasarathy Camasamudram; Norbert Nowotny; Erika Zilahi; Shawichi Iwamuro; Per F. Nielsen; Tibor Pál

doi:10.1016/S0006-291X(03)00999-9

A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties

J. Michael Conlon^*, Agnes Sonnevend, Mahendra Patel, Vijayayasarathy Camasamudram, Norbert Nowotny, Erika Zilahi, Shawichi Iwamuro, Per F. Nielsen, Tibor Pál

^*Corresponding author for this work

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Two peptides with antimicrobial and cytolytic properties were purified from an extract of the skin of Tago's brown frog Rana tagoi. The primary structure of one peptide (FLPILGKLLS¹⁰GIL·NH₂) identifies it as a member of the temporin family, whereas the second peptide (AIGSILGALA¹⁰KGLPTLISWI²⁰KNR·NH₂) displays 78% sequence identity to melittin from the venom of the honeybee Apis florea. Compared with melittin, the melittin-related peptide (MRP) was equipotent in inhibiting the growth of the Gram-positive bacterium Staphylococcus aureus, 5-fold less potent against the Gram-negative bacterium Escherichia coli and against the fungal pathogen, Candida albicans. MRP was 13-fold less hemolytic than melittin against human erythrocytes and 4- and 5-fold less cytolytic against mouse EL4 T-lymphoma-derived cells and L929 fibroblasts, respectively. However, at non-cytotoxic concentrations (≤8μM), MRP did not protect HeLa cells from cell death produced by human rhinovirus type 2 infection.

Original language	English
Pages (from-to)	496-500
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	306
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(03)00999-9
Publication status	Published - Jun 27 2003

Keywords

Antimicrobial
Antiviral
Cytolytic
Frog skin
Hemolytic
Melittin
Temporin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties. / Conlon, J. Michael; Sonnevend, Agnes; Patel, Mahendra; Camasamudram, Vijayayasarathy; Nowotny, Norbert; Zilahi, Erika; Iwamuro, Shawichi; Nielsen, Per F.; Pál, Tibor.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 2, 27.06.2003, p. 496-500.

Research output: Contribution to journal › Article › peer-review

Conlon, J. Michael ; Sonnevend, Agnes ; Patel, Mahendra ; Camasamudram, Vijayayasarathy ; Nowotny, Norbert ; Zilahi, Erika ; Iwamuro, Shawichi ; Nielsen, Per F. ; Pál, Tibor. / A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 2. pp. 496-500.

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abstract = "Two peptides with antimicrobial and cytolytic properties were purified from an extract of the skin of Tago's brown frog Rana tagoi. The primary structure of one peptide (FLPILGKLLS10GIL·NH2) identifies it as a member of the temporin family, whereas the second peptide (AIGSILGALA10KGLPTLISWI20KNR·NH2) displays 78% sequence identity to melittin from the venom of the honeybee Apis florea. Compared with melittin, the melittin-related peptide (MRP) was equipotent in inhibiting the growth of the Gram-positive bacterium Staphylococcus aureus, 5-fold less potent against the Gram-negative bacterium Escherichia coli and against the fungal pathogen, Candida albicans. MRP was 13-fold less hemolytic than melittin against human erythrocytes and 4- and 5-fold less cytolytic against mouse EL4 T-lymphoma-derived cells and L929 fibroblasts, respectively. However, at non-cytotoxic concentrations (≤8μM), MRP did not protect HeLa cells from cell death produced by human rhinovirus type 2 infection.",

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AU - Sonnevend, Agnes

AU - Patel, Mahendra

AU - Camasamudram, Vijayayasarathy

AU - Nowotny, Norbert

AU - Zilahi, Erika

AU - Iwamuro, Shawichi

AU - Nielsen, Per F.

AU - Pál, Tibor

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AB - Two peptides with antimicrobial and cytolytic properties were purified from an extract of the skin of Tago's brown frog Rana tagoi. The primary structure of one peptide (FLPILGKLLS10GIL·NH2) identifies it as a member of the temporin family, whereas the second peptide (AIGSILGALA10KGLPTLISWI20KNR·NH2) displays 78% sequence identity to melittin from the venom of the honeybee Apis florea. Compared with melittin, the melittin-related peptide (MRP) was equipotent in inhibiting the growth of the Gram-positive bacterium Staphylococcus aureus, 5-fold less potent against the Gram-negative bacterium Escherichia coli and against the fungal pathogen, Candida albicans. MRP was 13-fold less hemolytic than melittin against human erythrocytes and 4- and 5-fold less cytolytic against mouse EL4 T-lymphoma-derived cells and L929 fibroblasts, respectively. However, at non-cytotoxic concentrations (≤8μM), MRP did not protect HeLa cells from cell death produced by human rhinovirus type 2 infection.

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KW - Antiviral

KW - Cytolytic

KW - Frog skin

KW - Hemolytic

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KW - Temporin

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A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties

Abstract

Keywords

ASJC Scopus subject areas

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