A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties

J. Michael Conlon*, Agnes Sonnevend, Mahendra Patel, Vijayayasarathy Camasamudram, Norbert Nowotny, Erika Zilahi, Shawichi Iwamuro, Per F. Nielsen, Tibor Pál

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء

62 اقتباسات (Scopus)

ملخص

Two peptides with antimicrobial and cytolytic properties were purified from an extract of the skin of Tago's brown frog Rana tagoi. The primary structure of one peptide (FLPILGKLLS10GIL·NH2) identifies it as a member of the temporin family, whereas the second peptide (AIGSILGALA10KGLPTLISWI20KNR·NH2) displays 78% sequence identity to melittin from the venom of the honeybee Apis florea. Compared with melittin, the melittin-related peptide (MRP) was equipotent in inhibiting the growth of the Gram-positive bacterium Staphylococcus aureus, 5-fold less potent against the Gram-negative bacterium Escherichia coli and against the fungal pathogen, Candida albicans. MRP was 13-fold less hemolytic than melittin against human erythrocytes and 4- and 5-fold less cytolytic against mouse EL4 T-lymphoma-derived cells and L929 fibroblasts, respectively. However, at non-cytotoxic concentrations (≤8μM), MRP did not protect HeLa cells from cell death produced by human rhinovirus type 2 infection.

اللغة الأصليةEnglish
الصفحات (من إلى)496-500
عدد الصفحات5
دوريةBiochemical and Biophysical Research Communications
مستوى الصوت306
رقم الإصدار2
المعرِّفات الرقمية للأشياء
حالة النشرPublished - يونيو 27 2003
منشور خارجيًانعم

ASJC Scopus subject areas

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