TY - JOUR
T1 - Voltammetric behaviour of nitroxazepine in solubilized system and biological fluids
AU - Jain, Rajeev
AU - Rather, Jahangir Ahmad
AU - Dwivedi, Ashish
PY - 2011/3/12
Y1 - 2011/3/12
N2 - This study reports the development and validation of sensitive and selective assay method for the determination of the antidepressant drug in solubilized system and biological fluids. Solubilized system of different surfactants including cationic, anionic and non-ionic influences the electrochemical response of drug. Addition of cationic surfactant cetrimide to the solution containing drug enhances the peak current signal while anionic and non-ionic showed an opposite effect. The current signal due to reduction process was function of concentration of nitroxazepine, pH, type of surfactant and preconcentration time at the electrode surface. The reduction process is irreversible and adsorption controlled at HMDE. Various chemical and instrumental parameters affecting the monitored electroanalytical response were investigated and optimized for niroxazepine hydrochloride determination. The proposed SWCAdSV and DPCAdSV methods are linear over the concentration range 2.0 × 10-7- 5.0 × 10-9 mol/L and 6.1 × 10-7- 1.0 × 10-8 mol/L with detection limit of 1.62 × 10-10 mo/L and 1.4 × 10-9 mo/L respectively. The method shows good sensitivity, selectivity, accuracy and precision that makes it very suitable for determination of nitroxazepine in pharmaceutical formulation and biological fluids.
AB - This study reports the development and validation of sensitive and selective assay method for the determination of the antidepressant drug in solubilized system and biological fluids. Solubilized system of different surfactants including cationic, anionic and non-ionic influences the electrochemical response of drug. Addition of cationic surfactant cetrimide to the solution containing drug enhances the peak current signal while anionic and non-ionic showed an opposite effect. The current signal due to reduction process was function of concentration of nitroxazepine, pH, type of surfactant and preconcentration time at the electrode surface. The reduction process is irreversible and adsorption controlled at HMDE. Various chemical and instrumental parameters affecting the monitored electroanalytical response were investigated and optimized for niroxazepine hydrochloride determination. The proposed SWCAdSV and DPCAdSV methods are linear over the concentration range 2.0 × 10-7- 5.0 × 10-9 mol/L and 6.1 × 10-7- 1.0 × 10-8 mol/L with detection limit of 1.62 × 10-10 mo/L and 1.4 × 10-9 mo/L respectively. The method shows good sensitivity, selectivity, accuracy and precision that makes it very suitable for determination of nitroxazepine in pharmaceutical formulation and biological fluids.
KW - Adsorptive stripping voltammetry
KW - Biological fluids
KW - HMDE
KW - Nitroxazepine (Nitrox)
KW - Pharmaceutical formulation
KW - Solubilized system
UR - http://www.scopus.com/inward/record.url?scp=78650694303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650694303&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2010.09.001
DO - 10.1016/j.msec.2010.09.001
M3 - Article
AN - SCOPUS:78650694303
SN - 0928-4931
VL - 31
SP - 230
EP - 237
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
IS - 2
ER -