Metacaspase Deletion Increases Carbonylated and Tyrosine-Phosphorylated Proteins associated with Protein Synthesis and Carbohydrate Metabolism in Saccharomyces cerevisiae

Hydrogen peroxide (H 2 O 2) is an oxidant which could induce posttranslational modifications of proteins (PTMPs) in cells. It is still unknown that carbonylated proteins (CPs) were accumulated in caspase-suppressed leukemia cells or caspase-deleted Saccharomyces cerevisiae (yeast). Hence, we aimed to identify CPs and elucidate the role of metacaspase in regulating PTMPs and identify/compare the differentially expressed PTMPs in Δyca1 mutant compared to wild type with/without H 2 O 2 exposure by proteomics approach. We found that deletion of the metacaspase gene (MCG) in yeast resulted in accumulation of high amounts of PTMPs associated with protein synthesis and carbohydrate metabolism compared to H 2 O 2, which suggests that MCG is involved in the regulation of PTMPs and it could protect yeast from oxidative stress.