Metacaspase Deletion Increases Carbonylated and Tyrosine-Phosphorylated Proteins associated with Protein Synthesis and Carbohydrate Metabolism in Saccharomyces cerevisiae

Mohammed Abdul Khan*, Meghan Morgan, Musthafa Mohamed Essa, Mohammed Akbar, Gilles Guillemin, Byoung Joon Song

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Hydrogen peroxide (H 2 O 2) is an oxidant which could induce posttranslational modifications of proteins (PTMPs) in cells. It is still unknown that carbonylated proteins (CPs) were accumulated in caspase-suppressed leukemia cells or caspase-deleted Saccharomyces cerevisiae (yeast). Hence, we aimed to identify CPs and elucidate the role of metacaspase in regulating PTMPs and identify/compare the differentially expressed PTMPs in Δyca1 mutant compared to wild type with/without H 2 O 2 exposure by proteomics approach. We found that deletion of the metacaspase gene (MCG) in yeast resulted in accumulation of high amounts of PTMPs associated with protein synthesis and carbohydrate metabolism compared to H 2 O 2, which suggests that MCG is involved in the regulation of PTMPs and it could protect yeast from oxidative stress.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalInternational Journal of Nutrition, Pharmacology, Neurological Diseases
Volume12
Issue number2
DOIs
Publication statusPublished - Jan 1 2022

Keywords

  • carbonylation
  • metacaspase
  • proteome
  • tyrosine phosphorylation
  • yeast

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Clinical Neurology
  • Pharmacology (medical)

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