TY - JOUR
T1 - Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins
AU - Kalpana, Krishnan
AU - Maruthasalam, Subbiyan
AU - Rajesh, Thangaswamy
AU - Poovannan, Kandasami
AU - Kumar, Krish K.
AU - Kokiladevi, Easwaran
AU - Raja, Joseph A.J.
AU - Sudhakar, Durailagaraja
AU - Velazhahan, Rethinaswamy
AU - Samiyappan, Ramasamy
AU - Balasubramanian, Ponnuswami
N1 - Funding Information:
We are grateful to The Rockefeller Foundation, USA [# 99001/225 (PB) and # 99001/227 (DS)] and the Department of Biotechnology, New Delhi, India, for providing the financial support. We greatly acknowledge the help rendered by Dr. Legrand, Strasbourg, Cedex, France, and Dr. S. Muthukrishnan, Kansas State University, USA.
PY - 2006/2
Y1 - 2006/2
N2 - Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.
AB - Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.
KW - Chitinase
KW - Co-expression
KW - Defense genes
KW - Disease resistance
KW - Pathogenesis-related proteins
KW - Rice sheath blight
KW - Rice sheath rot
KW - Thaumatin-like protein
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U2 - 10.1016/j.plantsci.2005.08.002
DO - 10.1016/j.plantsci.2005.08.002
M3 - Article
AN - SCOPUS:29244436834
SN - 0168-9452
VL - 170
SP - 203
EP - 215
JO - Plant Science
JF - Plant Science
IS - 2
ER -