Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins

Krishnan Kalpana, Subbiyan Maruthasalam, Thangaswamy Rajesh, Kandasami Poovannan, Krish K. Kumar, Easwaran Kokiladevi, Joseph A.J. Raja, Durailagaraja Sudhakar, Rethinaswamy Velazhahan, Ramasamy Samiyappan, Ponnuswami Balasubramanian

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.

Original languageEnglish
Pages (from-to)203-215
Number of pages13
JournalPlant Science
Volume170
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

blight
engineering
transgenes
Transgenes
rice
cultivars
Genes
Thanatephorus cucumeris
Proteins
genes
proteins
Sarocladium oryzae
Western blotting
Western Blotting
pathogens
Rhizoctonia
Chitinases
chitinase
Southern blotting
inheritance (genetics)

Keywords

  • Chitinase
  • Co-expression
  • Defense genes
  • Disease resistance
  • Pathogenesis-related proteins
  • Rice sheath blight
  • Rice sheath rot
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Kalpana, K., Maruthasalam, S., Rajesh, T., Poovannan, K., Kumar, K. K., Kokiladevi, E., ... Balasubramanian, P. (2006). Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins. Plant Science, 170(2), 203-215. https://doi.org/10.1016/j.plantsci.2005.08.002

Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins. / Kalpana, Krishnan; Maruthasalam, Subbiyan; Rajesh, Thangaswamy; Poovannan, Kandasami; Kumar, Krish K.; Kokiladevi, Easwaran; Raja, Joseph A.J.; Sudhakar, Durailagaraja; Velazhahan, Rethinaswamy; Samiyappan, Ramasamy; Balasubramanian, Ponnuswami.

In: Plant Science, Vol. 170, No. 2, 02.2006, p. 203-215.

Research output: Contribution to journalArticle

Kalpana, K, Maruthasalam, S, Rajesh, T, Poovannan, K, Kumar, KK, Kokiladevi, E, Raja, JAJ, Sudhakar, D, Velazhahan, R, Samiyappan, R & Balasubramanian, P 2006, 'Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins', Plant Science, vol. 170, no. 2, pp. 203-215. https://doi.org/10.1016/j.plantsci.2005.08.002
Kalpana, Krishnan ; Maruthasalam, Subbiyan ; Rajesh, Thangaswamy ; Poovannan, Kandasami ; Kumar, Krish K. ; Kokiladevi, Easwaran ; Raja, Joseph A.J. ; Sudhakar, Durailagaraja ; Velazhahan, Rethinaswamy ; Samiyappan, Ramasamy ; Balasubramanian, Ponnuswami. / Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins. In: Plant Science. 2006 ; Vol. 170, No. 2. pp. 203-215.
@article{060147c10c574a0cad8d26ccb9b5e037,
title = "Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins",
abstract = "Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.",
keywords = "Chitinase, Co-expression, Defense genes, Disease resistance, Pathogenesis-related proteins, Rice sheath blight, Rice sheath rot, Thaumatin-like protein",
author = "Krishnan Kalpana and Subbiyan Maruthasalam and Thangaswamy Rajesh and Kandasami Poovannan and Kumar, {Krish K.} and Easwaran Kokiladevi and Raja, {Joseph A.J.} and Durailagaraja Sudhakar and Rethinaswamy Velazhahan and Ramasamy Samiyappan and Ponnuswami Balasubramanian",
year = "2006",
month = "2",
doi = "10.1016/j.plantsci.2005.08.002",
language = "English",
volume = "170",
pages = "203--215",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins

AU - Kalpana, Krishnan

AU - Maruthasalam, Subbiyan

AU - Rajesh, Thangaswamy

AU - Poovannan, Kandasami

AU - Kumar, Krish K.

AU - Kokiladevi, Easwaran

AU - Raja, Joseph A.J.

AU - Sudhakar, Durailagaraja

AU - Velazhahan, Rethinaswamy

AU - Samiyappan, Ramasamy

AU - Balasubramanian, Ponnuswami

PY - 2006/2

Y1 - 2006/2

N2 - Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.

AB - Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.

KW - Chitinase

KW - Co-expression

KW - Defense genes

KW - Disease resistance

KW - Pathogenesis-related proteins

KW - Rice sheath blight

KW - Rice sheath rot

KW - Thaumatin-like protein

UR - http://www.scopus.com/inward/record.url?scp=29244436834&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29244436834&partnerID=8YFLogxK

U2 - 10.1016/j.plantsci.2005.08.002

DO - 10.1016/j.plantsci.2005.08.002

M3 - Article

AN - SCOPUS:29244436834

VL - 170

SP - 203

EP - 215

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 2

ER -