Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins

Krishnan Kalpana, Subbiyan Maruthasalam, Thangaswamy Rajesh, Kandasami Poovannan, Krish K. Kumar, Easwaran Kokiladevi, Joseph A.J. Raja, Durailagaraja Sudhakar, Rethinaswamy Velazhahan, Ramasamy Samiyappan, Ponnuswami Balasubramanian

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47 Citations (Scopus)

Abstract

Genetically transformed lines of the elite indica rice cultivars, ADT38, ASD16, IR50 and Pusa Basmati1, constitutively over-expressing rice tlp encoding a thaumatin-like protein, have been evolved for management of rice sheath blight disease. The incorporation of tlp transgene in the genomes of T0 lines was ascertained by polymerase chain reaction and confirmed by Southern hybridization analyses. Expression of tlp in the putative transformants was confirmed by western blotting analysis. Stable inheritance of the transgene expression was studied up to T2 generation by western blotting analysis. The putative transformants and their progenies expressing tlp showed enhanced resistance against the sheath blight pathogen, Rhizoctonia solani, when compared to the non-transformed plants. The use of rice chi11, encoding a chitinase, as a co-transgene along with tlp produced a tlp-chi11 co-transformant that showed enhanced resistance against R. solani than the ones that express tlp or chi11 transgene alone. In addition to sheath blight resistance, the tlp or chi11 transgenic lines displayed significant levels of protection against the rice sheath rot pathogen, Sarocladium oryzae.

Original languageEnglish
Pages (from-to)203-215
Number of pages13
JournalPlant Science
Volume170
Issue number2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • Chitinase
  • Co-expression
  • Defense genes
  • Disease resistance
  • Pathogenesis-related proteins
  • Rice sheath blight
  • Rice sheath rot
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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  • Cite this

    Kalpana, K., Maruthasalam, S., Rajesh, T., Poovannan, K., Kumar, K. K., Kokiladevi, E., Raja, J. A. J., Sudhakar, D., Velazhahan, R., Samiyappan, R., & Balasubramanian, P. (2006). Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins. Plant Science, 170(2), 203-215. https://doi.org/10.1016/j.plantsci.2005.08.002