@article{d5c4a2c653844d4d92850baba58184c9,
title = "Diagnostic mr31/32000 Schistosoma mansoni proteins (sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium",
abstract = "Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n=123) and all but one from those with urinary schistosomiasis (n=35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are dose to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many 5. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.",
keywords = "Schistosoma haematobium, Schistosoma mansoni, Sm31/32, diagnostic proteins, immunoblots, patient sera, serodiagnosis",
author = "Idris, {Mohamed A.} and Andreas Ruppel",
note = "Funding Information: ACKNOWLEDGEMENTS We would like to thank the staff and children of Zuhal Elementary School, Abu Doun Junior Secondary School and Zakyab Elementary School for their indispensable cooperation; Dr. S. M. Suliman (Bilharzia Research Unit, Khartoum, Sudan) for invaluable help with infection of the monkeys; Dr. A. A. Daffalla (Blue Nile Health Project, Khartoum) and his colleagues for their logistic help; Dr. F. J. Ramalho-Pinto (University of Minas Gerais, Belo Horizonte, Brasil) for a generous gift of schistosomiasis sera; Dr. H. Guera (Universidad Peruana Cayetano Heredia, Lima, Peru) for kindly providing the fascioliasis sera; and Ms R. Hambeck (Institute for Immunology, University of Heidelberg) for expert technical assistance. This work was supported by a travel grant from the Deutscher Akademischer Austauschdienst to M.A.I, and grants from the Krebs-und Scharlachstiftung and the Forschungsschwerpunkt HD19 to A.R..",
year = "1988",
month = jun,
doi = "10.1017/S0022149X00011305",
language = "English",
volume = "62",
pages = "95--101",
journal = "Journal of Helminthology",
issn = "0022-149X",
publisher = "Cambridge University Press",
number = "2",
}