Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii

The antibiotics produced by the antagonistic Bacillus subtilis strain G-1 were partially purified and characterized. Thin-layer chromatography (TLC) analysis of the metabolites produced by B. subtilis G-1 showed a fluorescent compound at Relative front (R_f) 0.48 under UV light. The metabolite with R_f 0.48 purified from preparative TLC inhibited the mycelial growth of Sclerotium rolfsii in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis of the bioactive compound at R_f 0.48 revealed the presence of 22 different kinds of antibiotics which included aldehydes, fatty acids, alkanes, esters and sulphur containing compounds.