Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii

H. Shifa; C. Gopalakrishnan; R. Velazhahan

Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii

H. Shifa^*, C. Gopalakrishnan, R. Velazhahan

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The antibiotics produced by the antagonistic Bacillus subtilis strain G-1 were partially purified and characterized. Thin-layer chromatography (TLC) analysis of the metabolites produced by B. subtilis G-1 showed a fluorescent compound at Relative front (R_f) 0.48 under UV light. The metabolite with R_f 0.48 purified from preparative TLC inhibited the mycelial growth of Sclerotium rolfsii in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis of the bioactive compound at R_f 0.48 revealed the presence of 22 different kinds of antibiotics which included aldehydes, fatty acids, alkanes, esters and sulphur containing compounds.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Biochemical and Cellular Archives
Volume	15
Issue number	1
Publication status	Published - Apr 1 2015
Externally published	Yes

Keywords

Antibiotics
Bacillus subtilis
Biological control
Sclerotium rolfsii

ASJC Scopus subject areas

Biochemistry
Cell Biology

Cite this

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title = "Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii",

abstract = "The antibiotics produced by the antagonistic Bacillus subtilis strain G-1 were partially purified and characterized. Thin-layer chromatography (TLC) analysis of the metabolites produced by B. subtilis G-1 showed a fluorescent compound at Relative front (Rf) 0.48 under UV light. The metabolite with Rf 0.48 purified from preparative TLC inhibited the mycelial growth of Sclerotium rolfsii in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis of the bioactive compound at Rf 0.48 revealed the presence of 22 different kinds of antibiotics which included aldehydes, fatty acids, alkanes, esters and sulphur containing compounds.",

keywords = "Antibiotics, Bacillus subtilis, Biological control, Sclerotium rolfsii",

author = "H. Shifa and C. Gopalakrishnan and R. Velazhahan",

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T1 - Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii

AU - Shifa, H.

AU - Gopalakrishnan, C.

AU - Velazhahan, R.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - The antibiotics produced by the antagonistic Bacillus subtilis strain G-1 were partially purified and characterized. Thin-layer chromatography (TLC) analysis of the metabolites produced by B. subtilis G-1 showed a fluorescent compound at Relative front (Rf) 0.48 under UV light. The metabolite with Rf 0.48 purified from preparative TLC inhibited the mycelial growth of Sclerotium rolfsii in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis of the bioactive compound at Rf 0.48 revealed the presence of 22 different kinds of antibiotics which included aldehydes, fatty acids, alkanes, esters and sulphur containing compounds.

AB - The antibiotics produced by the antagonistic Bacillus subtilis strain G-1 were partially purified and characterized. Thin-layer chromatography (TLC) analysis of the metabolites produced by B. subtilis G-1 showed a fluorescent compound at Relative front (Rf) 0.48 under UV light. The metabolite with Rf 0.48 purified from preparative TLC inhibited the mycelial growth of Sclerotium rolfsii in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis of the bioactive compound at Rf 0.48 revealed the presence of 22 different kinds of antibiotics which included aldehydes, fatty acids, alkanes, esters and sulphur containing compounds.

KW - Antibiotics

KW - Bacillus subtilis

KW - Biological control

KW - Sclerotium rolfsii

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Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii

Abstract

Keywords

ASJC Scopus subject areas

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