Characterization of antifungal antibiotics produced by Bacillus subtilis G-1 antagonistic to Sclerotium rolfsii

H. Shifa*, C. Gopalakrishnan, R. Velazhahan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The antibiotics produced by the antagonistic Bacillus subtilis strain G-1 were partially purified and characterized. Thin-layer chromatography (TLC) analysis of the metabolites produced by B. subtilis G-1 showed a fluorescent compound at Relative front (Rf) 0.48 under UV light. The metabolite with Rf 0.48 purified from preparative TLC inhibited the mycelial growth of Sclerotium rolfsii in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis of the bioactive compound at Rf 0.48 revealed the presence of 22 different kinds of antibiotics which included aldehydes, fatty acids, alkanes, esters and sulphur containing compounds.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalBiochemical and Cellular Archives
Issue number1
Publication statusPublished - Apr 1 2015
Externally publishedYes


  • Antibiotics
  • Bacillus subtilis
  • Biological control
  • Sclerotium rolfsii

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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