Visualization of gene expression of short and long forms of prolactin receptor in rat digestive tissues

A. Ouhtit, P. A. Kelly, G. Morel

Research output: Contribution to journalArticle

Abstract

Several effects of prolactin have been characterized in various tissues of the gastrointestinal tract. In the present study, the expression of short and long forms of prolactin receptor was explored and quantified in the digestive tract and correlated to the prolactin specific functions. Sections of all digestive tissues were analyzed by in situ hybridization, using 35S- labeled oligoprobes unique to each form of receptor. Macroautoradiogram signals were quantified and expressed in arbitrary units. In rat liver, prolactin receptor mRNAs are expressed to a much greater degree in females than in males. The short-form transcript is significantly expressed to a greater degree in liver, whereas the long form predominates in the pancreas and esophagus. In the remainder of the gastrointestinal tract, there is an equivalent distribution of short- and long-form transcripts. Relatively high signal intensities are seen in the stomach, duodenum, jejunum, ileum, and colon, whereas the rectum is essentially negative. The identification of prolactin receptor gene expression to limited regions should help establish specific functions associated with this hormone in the digestive tissues.

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume266
Issue number5 29-5
Publication statusPublished - 1994

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Prolactin Receptors
Gastrointestinal Tract
Gene Expression
Prolactin
Liver
Jejunum
Ileum
Duodenum
Rectum
Esophagus
In Situ Hybridization
Pancreas
Stomach
Colon
Hormones
Messenger RNA

Keywords

  • in situ hybridization
  • messenger ribonucleic acid
  • quantification

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Visualization of gene expression of short and long forms of prolactin receptor in rat digestive tissues. / Ouhtit, A.; Kelly, P. A.; Morel, G.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 266, No. 5 29-5, 1994.

Research output: Contribution to journalArticle

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