TY - JOUR
T1 - Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia
AU - Mihara, Hiroshi
AU - Suzuki, Nobuhiro
AU - Boudaka, Ammar Abdullkader
AU - Muhammad, Jibran Sualeh
AU - Tominaga, Makoto
AU - Tabuchi, Yoshiaki
AU - Sugiyama, Toshiro
N1 - Publisher Copyright:
© The Author(s) 2016.
PY - 2016/6/28
Y1 - 2016/6/28
N2 - AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.
AB - AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.
KW - ATP
KW - Gastric emptying
KW - Stomach
KW - Transient receptor potential vanilloid 4
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U2 - 10.3748/wjg.v22.i24.5512
DO - 10.3748/wjg.v22.i24.5512
M3 - Article
C2 - 27350729
AN - SCOPUS:84979035695
SN - 1007-9327
VL - 22
SP - 5512
EP - 5519
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 24
ER -