Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Hiroshi Mihara, Nobuhiro Suzuki, Ammar Abdullkader Boudaka, Jibran Sualeh Muhammad, Makoto Tominaga, Yoshiaki Tabuchi, Toshiro Sugiyama

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

Original languageEnglish
Pages (from-to)5512-5519
Number of pages8
JournalWorld Journal of Gastroenterology
Volume22
Issue number24
DOIs
Publication statusPublished - Jun 28 2016

Fingerprint

TRPV Cation Channels
Stomach
Epithelium
Adenosine Triphosphate
Calcium
Gastric Emptying
Knockout Mice
Epithelial Cells
Immunochemistry
Messenger RNA
Electrophysiology
Luciferases
Proteins
Cell Line
Polymerase Chain Reaction

Keywords

  • ATP
  • Gastric emptying
  • Stomach
  • Transient receptor potential vanilloid 4

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia. / Mihara, Hiroshi; Suzuki, Nobuhiro; Boudaka, Ammar Abdullkader; Muhammad, Jibran Sualeh; Tominaga, Makoto; Tabuchi, Yoshiaki; Sugiyama, Toshiro.

In: World Journal of Gastroenterology, Vol. 22, No. 24, 28.06.2016, p. 5512-5519.

Research output: Contribution to journalArticle

Mihara, Hiroshi ; Suzuki, Nobuhiro ; Boudaka, Ammar Abdullkader ; Muhammad, Jibran Sualeh ; Tominaga, Makoto ; Tabuchi, Yoshiaki ; Sugiyama, Toshiro. / Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia. In: World Journal of Gastroenterology. 2016 ; Vol. 22, No. 24. pp. 5512-5519.
@article{de72734914a04426af04267c9c6a23ba,
title = "Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia",
abstract = "AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.",
keywords = "ATP, Gastric emptying, Stomach, Transient receptor potential vanilloid 4",
author = "Hiroshi Mihara and Nobuhiro Suzuki and Boudaka, {Ammar Abdullkader} and Muhammad, {Jibran Sualeh} and Makoto Tominaga and Yoshiaki Tabuchi and Toshiro Sugiyama",
year = "2016",
month = "6",
day = "28",
doi = "10.3748/wjg.v22.i24.5512",
language = "English",
volume = "22",
pages = "5512--5519",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "24",

}

TY - JOUR

T1 - Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

AU - Mihara, Hiroshi

AU - Suzuki, Nobuhiro

AU - Boudaka, Ammar Abdullkader

AU - Muhammad, Jibran Sualeh

AU - Tominaga, Makoto

AU - Tabuchi, Yoshiaki

AU - Sugiyama, Toshiro

PY - 2016/6/28

Y1 - 2016/6/28

N2 - AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

AB - AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia. METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice. RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo . CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.

KW - ATP

KW - Gastric emptying

KW - Stomach

KW - Transient receptor potential vanilloid 4

UR - http://www.scopus.com/inward/record.url?scp=84979035695&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979035695&partnerID=8YFLogxK

U2 - 10.3748/wjg.v22.i24.5512

DO - 10.3748/wjg.v22.i24.5512

M3 - Article

VL - 22

SP - 5512

EP - 5519

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 24

ER -