Toxicity of DTPA to Daphnia carinata as modified by oxygen stress and food limitation

R. A. Van Dam, M. J. Barry, J. T. Ahokas, D. A. Holdway

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

First-instar Daphnia carinata were exposed to one of four or five sublethal concentrations of the industrial chelating agent diethylenetriamine pentaacetic acid (DTPA) either alone, or in conjunction with, high (90-100%) or low (10-25%) oxygen saturation and high (2 x 105 cells/ml) or low (2 x 104 cells/ml) food conditions for 6 to 7 days, in a series of three experiments. Survival, growth, reproduction, and hemoglobin (Hb) content were assessed. Mortality increased significantly from 6.5 ± 4.2 to 38.9 ± 5.2%, and mean length was significantly reduced from 2.73 ± 0.02 to 1.37 ± 0.01 mm at 100 mg/liter DTPA in experiment 1. Mean length was also significantly reduced from 2.64 ± 0.12 to 1.9 ± 0.1 mm at 50 mg/liter DTPA in experiment 3. This was attributed to an indirect effect via the food supply in the third experiment. There was a significant decrease in the mean number of first- brood eggs at 10 mg/liter DTPA in all three experiments. Hemoglobin concentration was significantly increased under low oxygen conditions from 27.6 ± 1.7 to 65.5 ± 4.6 mg Hb/g Daphnia dry wt, and 23.0 ± 1.8 to 49.4 ± 3.5 mg Hb/g Daphnia dry wt in experiments 2 and 3, respectively. However, DTPA had no effect on hemoglobin concentration in any experiment. DTPA toxicity to D. carinata was not significantly altered by oxygen stress or food limitation and could not be attributed to an inhibition of Hb synthesis. Increased exposure times may result in further reproductive effects and also an indirect effect on hemoglobin concentration via the gradual depletion of iron stores. The no-observed effect concentration and the lowest observed effect concentration for D. carinata in this study were 1.0 and 10 mg/liter DTPA, respectively, based on reproduction, giving an estimated threshold concentration of 3.2 mg/liter DTPA.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalEcotoxicology and Environmental Safety
Volume31
Issue number2
DOIs
Publication statusPublished - 1995

Fingerprint

Daphnia
food limitation
Pentetic Acid
DTPA
Hemoglobin
hemoglobin
Toxicity
Hemoglobins
Oxygen
toxicity
Food
oxygen
Acids
experiment
Experiments
Reproduction
Food supply
chelating agent
Food Supply
dose-response relationship

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Pollution
  • Public Health, Environmental and Occupational Health

Cite this

Toxicity of DTPA to Daphnia carinata as modified by oxygen stress and food limitation. / Van Dam, R. A.; Barry, M. J.; Ahokas, J. T.; Holdway, D. A.

In: Ecotoxicology and Environmental Safety, Vol. 31, No. 2, 1995, p. 117-126.

Research output: Contribution to journalArticle

Van Dam, R. A. ; Barry, M. J. ; Ahokas, J. T. ; Holdway, D. A. / Toxicity of DTPA to Daphnia carinata as modified by oxygen stress and food limitation. In: Ecotoxicology and Environmental Safety. 1995 ; Vol. 31, No. 2. pp. 117-126.
@article{2ad04d958e40421f898a0e5f13bd18ea,
title = "Toxicity of DTPA to Daphnia carinata as modified by oxygen stress and food limitation",
abstract = "First-instar Daphnia carinata were exposed to one of four or five sublethal concentrations of the industrial chelating agent diethylenetriamine pentaacetic acid (DTPA) either alone, or in conjunction with, high (90-100{\%}) or low (10-25{\%}) oxygen saturation and high (2 x 105 cells/ml) or low (2 x 104 cells/ml) food conditions for 6 to 7 days, in a series of three experiments. Survival, growth, reproduction, and hemoglobin (Hb) content were assessed. Mortality increased significantly from 6.5 ± 4.2 to 38.9 ± 5.2{\%}, and mean length was significantly reduced from 2.73 ± 0.02 to 1.37 ± 0.01 mm at 100 mg/liter DTPA in experiment 1. Mean length was also significantly reduced from 2.64 ± 0.12 to 1.9 ± 0.1 mm at 50 mg/liter DTPA in experiment 3. This was attributed to an indirect effect via the food supply in the third experiment. There was a significant decrease in the mean number of first- brood eggs at 10 mg/liter DTPA in all three experiments. Hemoglobin concentration was significantly increased under low oxygen conditions from 27.6 ± 1.7 to 65.5 ± 4.6 mg Hb/g Daphnia dry wt, and 23.0 ± 1.8 to 49.4 ± 3.5 mg Hb/g Daphnia dry wt in experiments 2 and 3, respectively. However, DTPA had no effect on hemoglobin concentration in any experiment. DTPA toxicity to D. carinata was not significantly altered by oxygen stress or food limitation and could not be attributed to an inhibition of Hb synthesis. Increased exposure times may result in further reproductive effects and also an indirect effect on hemoglobin concentration via the gradual depletion of iron stores. The no-observed effect concentration and the lowest observed effect concentration for D. carinata in this study were 1.0 and 10 mg/liter DTPA, respectively, based on reproduction, giving an estimated threshold concentration of 3.2 mg/liter DTPA.",
author = "{Van Dam}, {R. A.} and Barry, {M. J.} and Ahokas, {J. T.} and Holdway, {D. A.}",
year = "1995",
doi = "10.1006/eesa.1995.1051",
language = "English",
volume = "31",
pages = "117--126",
journal = "Ecotoxicology and Environmental Safety",
issn = "0147-6513",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Toxicity of DTPA to Daphnia carinata as modified by oxygen stress and food limitation

AU - Van Dam, R. A.

AU - Barry, M. J.

AU - Ahokas, J. T.

AU - Holdway, D. A.

PY - 1995

Y1 - 1995

N2 - First-instar Daphnia carinata were exposed to one of four or five sublethal concentrations of the industrial chelating agent diethylenetriamine pentaacetic acid (DTPA) either alone, or in conjunction with, high (90-100%) or low (10-25%) oxygen saturation and high (2 x 105 cells/ml) or low (2 x 104 cells/ml) food conditions for 6 to 7 days, in a series of three experiments. Survival, growth, reproduction, and hemoglobin (Hb) content were assessed. Mortality increased significantly from 6.5 ± 4.2 to 38.9 ± 5.2%, and mean length was significantly reduced from 2.73 ± 0.02 to 1.37 ± 0.01 mm at 100 mg/liter DTPA in experiment 1. Mean length was also significantly reduced from 2.64 ± 0.12 to 1.9 ± 0.1 mm at 50 mg/liter DTPA in experiment 3. This was attributed to an indirect effect via the food supply in the third experiment. There was a significant decrease in the mean number of first- brood eggs at 10 mg/liter DTPA in all three experiments. Hemoglobin concentration was significantly increased under low oxygen conditions from 27.6 ± 1.7 to 65.5 ± 4.6 mg Hb/g Daphnia dry wt, and 23.0 ± 1.8 to 49.4 ± 3.5 mg Hb/g Daphnia dry wt in experiments 2 and 3, respectively. However, DTPA had no effect on hemoglobin concentration in any experiment. DTPA toxicity to D. carinata was not significantly altered by oxygen stress or food limitation and could not be attributed to an inhibition of Hb synthesis. Increased exposure times may result in further reproductive effects and also an indirect effect on hemoglobin concentration via the gradual depletion of iron stores. The no-observed effect concentration and the lowest observed effect concentration for D. carinata in this study were 1.0 and 10 mg/liter DTPA, respectively, based on reproduction, giving an estimated threshold concentration of 3.2 mg/liter DTPA.

AB - First-instar Daphnia carinata were exposed to one of four or five sublethal concentrations of the industrial chelating agent diethylenetriamine pentaacetic acid (DTPA) either alone, or in conjunction with, high (90-100%) or low (10-25%) oxygen saturation and high (2 x 105 cells/ml) or low (2 x 104 cells/ml) food conditions for 6 to 7 days, in a series of three experiments. Survival, growth, reproduction, and hemoglobin (Hb) content were assessed. Mortality increased significantly from 6.5 ± 4.2 to 38.9 ± 5.2%, and mean length was significantly reduced from 2.73 ± 0.02 to 1.37 ± 0.01 mm at 100 mg/liter DTPA in experiment 1. Mean length was also significantly reduced from 2.64 ± 0.12 to 1.9 ± 0.1 mm at 50 mg/liter DTPA in experiment 3. This was attributed to an indirect effect via the food supply in the third experiment. There was a significant decrease in the mean number of first- brood eggs at 10 mg/liter DTPA in all three experiments. Hemoglobin concentration was significantly increased under low oxygen conditions from 27.6 ± 1.7 to 65.5 ± 4.6 mg Hb/g Daphnia dry wt, and 23.0 ± 1.8 to 49.4 ± 3.5 mg Hb/g Daphnia dry wt in experiments 2 and 3, respectively. However, DTPA had no effect on hemoglobin concentration in any experiment. DTPA toxicity to D. carinata was not significantly altered by oxygen stress or food limitation and could not be attributed to an inhibition of Hb synthesis. Increased exposure times may result in further reproductive effects and also an indirect effect on hemoglobin concentration via the gradual depletion of iron stores. The no-observed effect concentration and the lowest observed effect concentration for D. carinata in this study were 1.0 and 10 mg/liter DTPA, respectively, based on reproduction, giving an estimated threshold concentration of 3.2 mg/liter DTPA.

UR - http://www.scopus.com/inward/record.url?scp=0029020408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029020408&partnerID=8YFLogxK

U2 - 10.1006/eesa.1995.1051

DO - 10.1006/eesa.1995.1051

M3 - Article

C2 - 8521776

AN - SCOPUS:0029020408

VL - 31

SP - 117

EP - 126

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

IS - 2

ER -