Therapeutic attenuation of neuroinflammation and apoptosis by black tea theaflavin in chronic MPTP/Probenecid model of parkinson's disease

Annadurai Anandhan, Musthafa Mohamed Essa, Thamilarasan Manivasagam

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Neuroinflammation and apoptosis in the dopaminergic neurons of substantia nigra play an important role in the pathogenesis of experimental and clinical Parkinson's disease (PD). This study focused on the possible anti-inflammatory and anti-apoptotic effects of theaflavin (TF), a black tea polyphenol against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity in mice. C57BL/6 male mice were treated with 10 doses of MPTP (25 mg/kg, s.c.) and probenecid (250 mg/kg, i.p.) for 3.5 days interval. TF (10 mg/kg) was administered 1 h prior to the administration of MPTP for 35 days of experimental period. MPTP/p treatment upregulates the release of interleukin-1beta, IL-6, tumor necrosis factor-alpha, IL-10, glial fibrillary acidic protein and Bax, and downregulates anti-apoptotic marker Bcl-2. Oral treatment of black tea polyphenol TF significantly attenuates MPTP-induced neuroinflammation as well as apoptosis. Behavioral studies (catalepsy and akinesia) were carried out to confirm these molecular studies. The results demonstrate that TF mediated its neuroprotection against chronic MPTP-induced toxicity through the involvement of multiple molecular events. It was concluded that TF may provide a precious therapeutic strategy for the treatment of progressive neurodegenerative disease such as PD in future.

Original languageEnglish
Pages (from-to)166-173
Number of pages8
JournalNeurotoxicity Research
Volume23
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Probenecid
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Tea
Parkinson Disease
Apoptosis
Polyphenols
Therapeutics
Neurodegenerative diseases
Catalepsy
Dopaminergic Neurons
Glial Fibrillary Acidic Protein
Parkinsonian Disorders
Substantia Nigra
Interleukin-1beta
Neurodegenerative Diseases
Interleukin-10
Neurons
Toxicity
theaflavin
Interleukin-6

Keywords

  • Apoptosis
  • Behavior
  • MPTP
  • Neuroinflammation
  • Parkinson's disease
  • Theaflavin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Therapeutic attenuation of neuroinflammation and apoptosis by black tea theaflavin in chronic MPTP/Probenecid model of parkinson's disease. / Anandhan, Annadurai; Essa, Musthafa Mohamed; Manivasagam, Thamilarasan.

In: Neurotoxicity Research, Vol. 23, No. 2, 2013, p. 166-173.

Research output: Contribution to journalArticle

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