The role of NK cells during in vivo antigen-specific antibody responses

Julie A. Wilder, Crystal Y. Koh, Dorothy Yuan

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Investigations into the role of NK cells in regulating Ab responses have yielded variable results, some suggesting that NK cells can down-regulate Ag- specific Ig production and others proposing an enhancing effect. These apparently inconsistent findings may stem partially from the specificity of reagents used in purifying cell populations and/or the nature of the in vitro systems used to study these events. We chose to investigate the ability of either resting or poly(I:C)-activated NK cells to alter an in vivo Ab response in mice given a T-independent (TNP-LPS) or T-dependent (TNP-keyhole limpet hemocyanin (KLH)) Ag. By using a more specific Ab, anti-NK-1.1, to deplete NK cells, we were able to clearly show that resting, endogenous NK cells do not affect either type of response, as measured by serum Ag-specific Ig levels quantitated by isotype-specific ELISA. In contrast, activation of NK cells by poly(I:C) increased Ag-specific IgG2a as well as IgG1 levels. Interestingly, only the effect on IgG2a production is reversible by depletion of NK cells.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalJournal of Immunology
Volume156
Issue number1
Publication statusPublished - Jan 1 1996

Fingerprint

Natural Killer Cells
Antibody Formation
Antigens
Down-Regulation
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Serum
Population

ASJC Scopus subject areas

  • Immunology

Cite this

The role of NK cells during in vivo antigen-specific antibody responses. / Wilder, Julie A.; Koh, Crystal Y.; Yuan, Dorothy.

In: Journal of Immunology, Vol. 156, No. 1, 01.01.1996, p. 146-152.

Research output: Contribution to journalArticle

Wilder, Julie A. ; Koh, Crystal Y. ; Yuan, Dorothy. / The role of NK cells during in vivo antigen-specific antibody responses. In: Journal of Immunology. 1996 ; Vol. 156, No. 1. pp. 146-152.
@article{1f50ac87738b40c0b7163eb6fa998ae9,
title = "The role of NK cells during in vivo antigen-specific antibody responses",
abstract = "Investigations into the role of NK cells in regulating Ab responses have yielded variable results, some suggesting that NK cells can down-regulate Ag- specific Ig production and others proposing an enhancing effect. These apparently inconsistent findings may stem partially from the specificity of reagents used in purifying cell populations and/or the nature of the in vitro systems used to study these events. We chose to investigate the ability of either resting or poly(I:C)-activated NK cells to alter an in vivo Ab response in mice given a T-independent (TNP-LPS) or T-dependent (TNP-keyhole limpet hemocyanin (KLH)) Ag. By using a more specific Ab, anti-NK-1.1, to deplete NK cells, we were able to clearly show that resting, endogenous NK cells do not affect either type of response, as measured by serum Ag-specific Ig levels quantitated by isotype-specific ELISA. In contrast, activation of NK cells by poly(I:C) increased Ag-specific IgG2a as well as IgG1 levels. Interestingly, only the effect on IgG2a production is reversible by depletion of NK cells.",
author = "Wilder, {Julie A.} and Koh, {Crystal Y.} and Dorothy Yuan",
year = "1996",
month = "1",
day = "1",
language = "English",
volume = "156",
pages = "146--152",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "1",

}

TY - JOUR

T1 - The role of NK cells during in vivo antigen-specific antibody responses

AU - Wilder, Julie A.

AU - Koh, Crystal Y.

AU - Yuan, Dorothy

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Investigations into the role of NK cells in regulating Ab responses have yielded variable results, some suggesting that NK cells can down-regulate Ag- specific Ig production and others proposing an enhancing effect. These apparently inconsistent findings may stem partially from the specificity of reagents used in purifying cell populations and/or the nature of the in vitro systems used to study these events. We chose to investigate the ability of either resting or poly(I:C)-activated NK cells to alter an in vivo Ab response in mice given a T-independent (TNP-LPS) or T-dependent (TNP-keyhole limpet hemocyanin (KLH)) Ag. By using a more specific Ab, anti-NK-1.1, to deplete NK cells, we were able to clearly show that resting, endogenous NK cells do not affect either type of response, as measured by serum Ag-specific Ig levels quantitated by isotype-specific ELISA. In contrast, activation of NK cells by poly(I:C) increased Ag-specific IgG2a as well as IgG1 levels. Interestingly, only the effect on IgG2a production is reversible by depletion of NK cells.

AB - Investigations into the role of NK cells in regulating Ab responses have yielded variable results, some suggesting that NK cells can down-regulate Ag- specific Ig production and others proposing an enhancing effect. These apparently inconsistent findings may stem partially from the specificity of reagents used in purifying cell populations and/or the nature of the in vitro systems used to study these events. We chose to investigate the ability of either resting or poly(I:C)-activated NK cells to alter an in vivo Ab response in mice given a T-independent (TNP-LPS) or T-dependent (TNP-keyhole limpet hemocyanin (KLH)) Ag. By using a more specific Ab, anti-NK-1.1, to deplete NK cells, we were able to clearly show that resting, endogenous NK cells do not affect either type of response, as measured by serum Ag-specific Ig levels quantitated by isotype-specific ELISA. In contrast, activation of NK cells by poly(I:C) increased Ag-specific IgG2a as well as IgG1 levels. Interestingly, only the effect on IgG2a production is reversible by depletion of NK cells.

UR - http://www.scopus.com/inward/record.url?scp=0030024044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030024044&partnerID=8YFLogxK

M3 - Article

VL - 156

SP - 146

EP - 152

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 1

ER -