The effect of citrullus colocynthis pulp extract on the liver of diabetic rats a light and scanning electron microscopic study

Mohammad Khalil, Gamal Mohamed, Mohammad Dallak, Fahaid Al-Hashem, Hussein Sakr, Refaat A. Eid, Mohamed A. Adly, Mahmoud Al-Khateeb, Saleh Banihani, Zuhair Hassan, Nabil Bashir

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Problem statement: The goal of the current investigation was to clarify the effects of Citrullus colocynthis pulp extract on the structure of the liver of diabetic rats at both light and scanning electron microscopic levels. Approach: Forty-eight adult male albino rats were equally allocated into four groups: Group1: control, Group 2: Citrullus colocynthis-treated, Group 3: diabetic rats and Group4: diabetic rats treated with Citrullus colocynthis. All treatments were administered via an intragastric tube. Diabetes was induced in the rats of groups 3 and 4 by an intraperitoneal injection with alloxan. Results: The liver of Citrullus colocynthis-treated rats revealed minor histological changes versus the control animals. In group 3 animals, diabetes caused degenerative alterations in the form of disorganization of the hepatic cords, cytoplasmic vacuolization and pyknosis of the nuclei of hepatocytes and inflammatory cell infiltration. Scanning electron microscope examination of these livers revealed numerous lipid droplets within hepatocytes, damaged blood sinusoids and hemorrhage of erythrocytes between hepatocytes and inside Disse's spaces. On the other hand, the normal histological and scanning ultrastructural features were nearly resumed in the liver of diabetic rats treated with Citrullus colocynthis pulp extract. Conclusion: The present study proved a lessening effect of Citrullus colocynthis pulp extract on the liver of diabetic rats. In light of these advantageous influences, it is advisable to widen the scale of its use in a trial to alleviate the diabetic hepatic adverse effects.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalAmerican Journal of Biochemistry and Biotechnology
Volume6
Issue number3
Publication statusPublished - 2010

Fingerprint

Liver Extracts
Liver
Pulp
Rats
Electrons
Scanning
Light
Hepatocytes
Medical problems
Animals
Alloxan
Citrullus colocynthis
Intraperitoneal Injections
Infiltration
Blood
Electron microscopes
Erythrocytes
Hemorrhage
Lipids
Control Groups

Keywords

  • Alloxan
  • Citrullus colocynthis
  • Diabetes
  • Liver
  • Rats

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry

Cite this

The effect of citrullus colocynthis pulp extract on the liver of diabetic rats a light and scanning electron microscopic study. / Khalil, Mohammad; Mohamed, Gamal; Dallak, Mohammad; Al-Hashem, Fahaid; Sakr, Hussein; Eid, Refaat A.; Adly, Mohamed A.; Al-Khateeb, Mahmoud; Banihani, Saleh; Hassan, Zuhair; Bashir, Nabil.

In: American Journal of Biochemistry and Biotechnology, Vol. 6, No. 3, 2010, p. 155-163.

Research output: Contribution to journalArticle

Khalil, M, Mohamed, G, Dallak, M, Al-Hashem, F, Sakr, H, Eid, RA, Adly, MA, Al-Khateeb, M, Banihani, S, Hassan, Z & Bashir, N 2010, 'The effect of citrullus colocynthis pulp extract on the liver of diabetic rats a light and scanning electron microscopic study', American Journal of Biochemistry and Biotechnology, vol. 6, no. 3, pp. 155-163.
Khalil, Mohammad ; Mohamed, Gamal ; Dallak, Mohammad ; Al-Hashem, Fahaid ; Sakr, Hussein ; Eid, Refaat A. ; Adly, Mohamed A. ; Al-Khateeb, Mahmoud ; Banihani, Saleh ; Hassan, Zuhair ; Bashir, Nabil. / The effect of citrullus colocynthis pulp extract on the liver of diabetic rats a light and scanning electron microscopic study. In: American Journal of Biochemistry and Biotechnology. 2010 ; Vol. 6, No. 3. pp. 155-163.
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AU - Eid, Refaat A.

AU - Adly, Mohamed A.

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AU - Hassan, Zuhair

AU - Bashir, Nabil

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N2 - Problem statement: The goal of the current investigation was to clarify the effects of Citrullus colocynthis pulp extract on the structure of the liver of diabetic rats at both light and scanning electron microscopic levels. Approach: Forty-eight adult male albino rats were equally allocated into four groups: Group1: control, Group 2: Citrullus colocynthis-treated, Group 3: diabetic rats and Group4: diabetic rats treated with Citrullus colocynthis. All treatments were administered via an intragastric tube. Diabetes was induced in the rats of groups 3 and 4 by an intraperitoneal injection with alloxan. Results: The liver of Citrullus colocynthis-treated rats revealed minor histological changes versus the control animals. In group 3 animals, diabetes caused degenerative alterations in the form of disorganization of the hepatic cords, cytoplasmic vacuolization and pyknosis of the nuclei of hepatocytes and inflammatory cell infiltration. Scanning electron microscope examination of these livers revealed numerous lipid droplets within hepatocytes, damaged blood sinusoids and hemorrhage of erythrocytes between hepatocytes and inside Disse's spaces. On the other hand, the normal histological and scanning ultrastructural features were nearly resumed in the liver of diabetic rats treated with Citrullus colocynthis pulp extract. Conclusion: The present study proved a lessening effect of Citrullus colocynthis pulp extract on the liver of diabetic rats. In light of these advantageous influences, it is advisable to widen the scale of its use in a trial to alleviate the diabetic hepatic adverse effects.

AB - Problem statement: The goal of the current investigation was to clarify the effects of Citrullus colocynthis pulp extract on the structure of the liver of diabetic rats at both light and scanning electron microscopic levels. Approach: Forty-eight adult male albino rats were equally allocated into four groups: Group1: control, Group 2: Citrullus colocynthis-treated, Group 3: diabetic rats and Group4: diabetic rats treated with Citrullus colocynthis. All treatments were administered via an intragastric tube. Diabetes was induced in the rats of groups 3 and 4 by an intraperitoneal injection with alloxan. Results: The liver of Citrullus colocynthis-treated rats revealed minor histological changes versus the control animals. In group 3 animals, diabetes caused degenerative alterations in the form of disorganization of the hepatic cords, cytoplasmic vacuolization and pyknosis of the nuclei of hepatocytes and inflammatory cell infiltration. Scanning electron microscope examination of these livers revealed numerous lipid droplets within hepatocytes, damaged blood sinusoids and hemorrhage of erythrocytes between hepatocytes and inside Disse's spaces. On the other hand, the normal histological and scanning ultrastructural features were nearly resumed in the liver of diabetic rats treated with Citrullus colocynthis pulp extract. Conclusion: The present study proved a lessening effect of Citrullus colocynthis pulp extract on the liver of diabetic rats. In light of these advantageous influences, it is advisable to widen the scale of its use in a trial to alleviate the diabetic hepatic adverse effects.

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