The application of 7-chloro-4-nitrobenzoxadiazole (NBD-Cl) for the analysis of pharmaceutical-bearing amine group using spectrophotometry and spectrofluorimetry techniques

Abdalla A. Elbashir, F. E O Suliman, Hassan Y. Aboul-Enein

Research output: Contribution to journalArticle

15 Citations (Scopus)


Many papers have been presented in recent years regarding the field of application of 7-chloro-4-nitrobenzoxadiazole (NBD-Cl) as a fluorogenic and chromogenic reagent for the determination of pharmaceutical amines using spectrophotometry and spectrofluorimetry techniques. In this review article, various spectrophotometric and spectrofluorimetric methods using NBD-Cl as a labeling reagent for determination of pharmaceutical amines are covered. The application of these methods for the determination of drugs in pharmaceutical and real samples is also discussed.

Original languageEnglish
Pages (from-to)222-241
Number of pages20
JournalApplied Spectroscopy Reviews
Issue number3
Publication statusPublished - Apr 2011



  • 7-Chloro-4-nitrobenzoxadiazole (NBD-Cl)
  • chromogenic spectrophotometry
  • fluorogenic
  • pharmaceutical amines
  • spectrofluorimetry

ASJC Scopus subject areas

  • Spectroscopy
  • Instrumentation

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