Terbium sensitized luminescence for the determination of ketoprofen in pharmaceutical formulations

Salma M Z Al-Kindy, Zakiya Al-Harasi, Fakhr Eldin O Suliman, Abdalla Al-Hamadi, Avin Pillay

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This paper explores an ultra-sensitive luminescence method for the determination of Ketoprofen (KP) in pharmaceutical formulations. The technique is indirect and exploits the luminescence enhancement of terbium (Tb 3+) by complexation with KP (Tb 3+-KP), which was monitored at respective excitation and emission wavelengths of λ ex=258 nm and λ em=549 nm. The effect of varying the Tb 3+ concentration and using multiple solvents was examined to determine optimal experimental conditions. Maximum sensitization was accomplished in the presence of methanol where the most favourable condition for the formation of the complex was recorded at a level of 1.0×10 -5 M of Tb 3+. Under these optimum experimental conditions, linear calibration curve was obtained in the range of 2.8×10 -7-3.1×10 -6 M with a detection limit of 8.7×10 -8 M. The technique was validated with 'working' reference standards and produced relative standard deviations <2% indicating that the reproducibility was highly acceptable. The proposed method was successfully applied to assays of KP in pharmaceutical formulations with average recoveries of 92-98%. The results were found to be in good agreement with those obtained by HPLC. The method is highly suited for general applications of this nature.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalJournal of Fluorescence
Volume19
Issue number2
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Terbium
Ketoprofen
Drug Compounding
Luminescence
pharmaceutical
Pharmaceutical Preparations
sensitization
Complexation
Calibration
Methanol
Limit of Detection
Assays
High Pressure Liquid Chromatography
Recovery
Wavelength

Keywords

  • Drug formulations
  • HPLC
  • Ketoprofen
  • Sensitized luminescence
  • Terbium

ASJC Scopus subject areas

  • Spectroscopy
  • Biochemistry
  • Clinical Biochemistry

Cite this

Terbium sensitized luminescence for the determination of ketoprofen in pharmaceutical formulations. / Al-Kindy, Salma M Z; Al-Harasi, Zakiya; Suliman, Fakhr Eldin O; Al-Hamadi, Abdalla; Pillay, Avin.

In: Journal of Fluorescence, Vol. 19, No. 2, 03.2009, p. 249-255.

Research output: Contribution to journalArticle

@article{bccb9ea5064946ee9d546bbb511b2341,
title = "Terbium sensitized luminescence for the determination of ketoprofen in pharmaceutical formulations",
abstract = "This paper explores an ultra-sensitive luminescence method for the determination of Ketoprofen (KP) in pharmaceutical formulations. The technique is indirect and exploits the luminescence enhancement of terbium (Tb 3+) by complexation with KP (Tb 3+-KP), which was monitored at respective excitation and emission wavelengths of λ ex=258 nm and λ em=549 nm. The effect of varying the Tb 3+ concentration and using multiple solvents was examined to determine optimal experimental conditions. Maximum sensitization was accomplished in the presence of methanol where the most favourable condition for the formation of the complex was recorded at a level of 1.0×10 -5 M of Tb 3+. Under these optimum experimental conditions, linear calibration curve was obtained in the range of 2.8×10 -7-3.1×10 -6 M with a detection limit of 8.7×10 -8 M. The technique was validated with 'working' reference standards and produced relative standard deviations <2{\%} indicating that the reproducibility was highly acceptable. The proposed method was successfully applied to assays of KP in pharmaceutical formulations with average recoveries of 92-98{\%}. The results were found to be in good agreement with those obtained by HPLC. The method is highly suited for general applications of this nature.",
keywords = "Drug formulations, HPLC, Ketoprofen, Sensitized luminescence, Terbium",
author = "Al-Kindy, {Salma M Z} and Zakiya Al-Harasi and Suliman, {Fakhr Eldin O} and Abdalla Al-Hamadi and Avin Pillay",
year = "2009",
month = "3",
doi = "10.1007/s10895-008-0410-6",
language = "English",
volume = "19",
pages = "249--255",
journal = "Journal of Fluorescence",
issn = "1053-0509",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Terbium sensitized luminescence for the determination of ketoprofen in pharmaceutical formulations

AU - Al-Kindy, Salma M Z

AU - Al-Harasi, Zakiya

AU - Suliman, Fakhr Eldin O

AU - Al-Hamadi, Abdalla

AU - Pillay, Avin

PY - 2009/3

Y1 - 2009/3

N2 - This paper explores an ultra-sensitive luminescence method for the determination of Ketoprofen (KP) in pharmaceutical formulations. The technique is indirect and exploits the luminescence enhancement of terbium (Tb 3+) by complexation with KP (Tb 3+-KP), which was monitored at respective excitation and emission wavelengths of λ ex=258 nm and λ em=549 nm. The effect of varying the Tb 3+ concentration and using multiple solvents was examined to determine optimal experimental conditions. Maximum sensitization was accomplished in the presence of methanol where the most favourable condition for the formation of the complex was recorded at a level of 1.0×10 -5 M of Tb 3+. Under these optimum experimental conditions, linear calibration curve was obtained in the range of 2.8×10 -7-3.1×10 -6 M with a detection limit of 8.7×10 -8 M. The technique was validated with 'working' reference standards and produced relative standard deviations <2% indicating that the reproducibility was highly acceptable. The proposed method was successfully applied to assays of KP in pharmaceutical formulations with average recoveries of 92-98%. The results were found to be in good agreement with those obtained by HPLC. The method is highly suited for general applications of this nature.

AB - This paper explores an ultra-sensitive luminescence method for the determination of Ketoprofen (KP) in pharmaceutical formulations. The technique is indirect and exploits the luminescence enhancement of terbium (Tb 3+) by complexation with KP (Tb 3+-KP), which was monitored at respective excitation and emission wavelengths of λ ex=258 nm and λ em=549 nm. The effect of varying the Tb 3+ concentration and using multiple solvents was examined to determine optimal experimental conditions. Maximum sensitization was accomplished in the presence of methanol where the most favourable condition for the formation of the complex was recorded at a level of 1.0×10 -5 M of Tb 3+. Under these optimum experimental conditions, linear calibration curve was obtained in the range of 2.8×10 -7-3.1×10 -6 M with a detection limit of 8.7×10 -8 M. The technique was validated with 'working' reference standards and produced relative standard deviations <2% indicating that the reproducibility was highly acceptable. The proposed method was successfully applied to assays of KP in pharmaceutical formulations with average recoveries of 92-98%. The results were found to be in good agreement with those obtained by HPLC. The method is highly suited for general applications of this nature.

KW - Drug formulations

KW - HPLC

KW - Ketoprofen

KW - Sensitized luminescence

KW - Terbium

UR - http://www.scopus.com/inward/record.url?scp=65549124986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549124986&partnerID=8YFLogxK

U2 - 10.1007/s10895-008-0410-6

DO - 10.1007/s10895-008-0410-6

M3 - Article

C2 - 18773282

AN - SCOPUS:65549124986

VL - 19

SP - 249

EP - 255

JO - Journal of Fluorescence

JF - Journal of Fluorescence

SN - 1053-0509

IS - 2

ER -