Serological, cultural and molecular evidence of Brucella melitensis infection in goats in Al Jabal Al Akhdar, Sultanate of Oman

Yasmin ElTahir, Al Ghalya Al Toobi, Waleed Al Marzooqi, Osman Gaafar, Maryne Jay, Yannick Corde, Hadi Al Lawati, Shekar Bose, Abeer Al Hamrashdi, Kaadhia Al Kharousi, Noor Alhuda Al Saqri, Rudaina Al Busaidi, Eugene H. Johnson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Brucellosis, one of the most common zoonotic diseases and has significant public health and economic importance worldwide. Few studies and reports have been performed to estimate the true prevalence of animal brucellosis in the Sultanate of Oman; however, no incidence of the disease was previously reported in Al Jabal Al Akhdar. The purpose of this study was to investigate the prevalence of brucellosis in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah, Al Jabal Al Akhdar in the Sultanate of Oman. In this study we used different diagnostic serological tests, namely, RBT, I-ELISA and CFT to study the prevalence of Brucella infection in goats in Al Jabal Al Akhdar. Statistical analysis using Kappa statistics was used to compare the performance of the serological tests. Biochemical tests and species-specific Multiplex PCR were used to identify the brucella species involved in the infection. A structured questionnaire and Chi-square (x2) statistical analysis was used to identify related brucellosis risk factors. This study is the first to reveal brucellosis infection in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah, with an overall seroprevalence of 11.1%. The study also compared the performance of three different serological tests, namely, RBT, I-ELISA and CFT. Statistical analysis using Kappa statistics showed that the degree of agreement was best seen between RBT and CFT (96%), followed by RBT, I- ELISA (91.4%) and CFT and I- ELISA (89.2%). Biochemical tests and species-specific Multiplex PCR showed the typical profile for B. melitensis. A structured questionnaire and Chi-square (x2) statistical analysis indicated that the presence of abortion is the major risk factor for the prevalence of brucellosis, whereas age and sex were not significant factors in the tested animals. Besides, poor knowledge about brucellosis, consumption of unpasteurized milk or milk products, free trade of animals and the introduction of new animal breeds to herds were all contributing risk factors to the prevalence of brucellosis. The prevalence of human brucellosis obtained verbally from pastoralists gave an insight that brucellosis could pose a public health hazard, especially in those high-risk groups, mainly the pastoralists in the study area. Because of their constant and increasing interaction with their animals, pastoralists could be at a high risk of occupational infection.

Original languageEnglish
Pages (from-to)190-205
Number of pages16
JournalVeterinary Medicine and Science
Volume4
Issue number3
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Brucella melitensis
Oman
Brucellosis
brucellosis
Goats
goats
Infection
infection
Serologic Tests
immunologic techniques
statistical analysis
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
hail
Brucella
risk factors
Multiplex Polymerase Chain Reaction
animals
villages
public health

Keywords

  • Al Jabal Al Akhdar
  • Brucella
  • Brucellosis
  • Oman

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Serological, cultural and molecular evidence of Brucella melitensis infection in goats in Al Jabal Al Akhdar, Sultanate of Oman. / ElTahir, Yasmin; Al Toobi, Al Ghalya; Al Marzooqi, Waleed; Gaafar, Osman; Jay, Maryne; Corde, Yannick; Al Lawati, Hadi; Bose, Shekar; Al Hamrashdi, Abeer; Al Kharousi, Kaadhia; Al Saqri, Noor Alhuda; Al Busaidi, Rudaina; H. Johnson, Eugene.

In: Veterinary Medicine and Science, Vol. 4, No. 3, 01.08.2018, p. 190-205.

Research output: Contribution to journalArticle

ElTahir, Yasmin ; Al Toobi, Al Ghalya ; Al Marzooqi, Waleed ; Gaafar, Osman ; Jay, Maryne ; Corde, Yannick ; Al Lawati, Hadi ; Bose, Shekar ; Al Hamrashdi, Abeer ; Al Kharousi, Kaadhia ; Al Saqri, Noor Alhuda ; Al Busaidi, Rudaina ; H. Johnson, Eugene. / Serological, cultural and molecular evidence of Brucella melitensis infection in goats in Al Jabal Al Akhdar, Sultanate of Oman. In: Veterinary Medicine and Science. 2018 ; Vol. 4, No. 3. pp. 190-205.
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T1 - Serological, cultural and molecular evidence of Brucella melitensis infection in goats in Al Jabal Al Akhdar, Sultanate of Oman

AU - ElTahir, Yasmin

AU - Al Toobi, Al Ghalya

AU - Al Marzooqi, Waleed

AU - Gaafar, Osman

AU - Jay, Maryne

AU - Corde, Yannick

AU - Al Lawati, Hadi

AU - Bose, Shekar

AU - Al Hamrashdi, Abeer

AU - Al Kharousi, Kaadhia

AU - Al Saqri, Noor Alhuda

AU - Al Busaidi, Rudaina

AU - H. Johnson, Eugene

PY - 2018/8/1

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N2 - Brucellosis, one of the most common zoonotic diseases and has significant public health and economic importance worldwide. Few studies and reports have been performed to estimate the true prevalence of animal brucellosis in the Sultanate of Oman; however, no incidence of the disease was previously reported in Al Jabal Al Akhdar. The purpose of this study was to investigate the prevalence of brucellosis in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah, Al Jabal Al Akhdar in the Sultanate of Oman. In this study we used different diagnostic serological tests, namely, RBT, I-ELISA and CFT to study the prevalence of Brucella infection in goats in Al Jabal Al Akhdar. Statistical analysis using Kappa statistics was used to compare the performance of the serological tests. Biochemical tests and species-specific Multiplex PCR were used to identify the brucella species involved in the infection. A structured questionnaire and Chi-square (x2) statistical analysis was used to identify related brucellosis risk factors. This study is the first to reveal brucellosis infection in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah, with an overall seroprevalence of 11.1%. The study also compared the performance of three different serological tests, namely, RBT, I-ELISA and CFT. Statistical analysis using Kappa statistics showed that the degree of agreement was best seen between RBT and CFT (96%), followed by RBT, I- ELISA (91.4%) and CFT and I- ELISA (89.2%). Biochemical tests and species-specific Multiplex PCR showed the typical profile for B. melitensis. A structured questionnaire and Chi-square (x2) statistical analysis indicated that the presence of abortion is the major risk factor for the prevalence of brucellosis, whereas age and sex were not significant factors in the tested animals. Besides, poor knowledge about brucellosis, consumption of unpasteurized milk or milk products, free trade of animals and the introduction of new animal breeds to herds were all contributing risk factors to the prevalence of brucellosis. The prevalence of human brucellosis obtained verbally from pastoralists gave an insight that brucellosis could pose a public health hazard, especially in those high-risk groups, mainly the pastoralists in the study area. Because of their constant and increasing interaction with their animals, pastoralists could be at a high risk of occupational infection.

AB - Brucellosis, one of the most common zoonotic diseases and has significant public health and economic importance worldwide. Few studies and reports have been performed to estimate the true prevalence of animal brucellosis in the Sultanate of Oman; however, no incidence of the disease was previously reported in Al Jabal Al Akhdar. The purpose of this study was to investigate the prevalence of brucellosis in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah, Al Jabal Al Akhdar in the Sultanate of Oman. In this study we used different diagnostic serological tests, namely, RBT, I-ELISA and CFT to study the prevalence of Brucella infection in goats in Al Jabal Al Akhdar. Statistical analysis using Kappa statistics was used to compare the performance of the serological tests. Biochemical tests and species-specific Multiplex PCR were used to identify the brucella species involved in the infection. A structured questionnaire and Chi-square (x2) statistical analysis was used to identify related brucellosis risk factors. This study is the first to reveal brucellosis infection in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah, with an overall seroprevalence of 11.1%. The study also compared the performance of three different serological tests, namely, RBT, I-ELISA and CFT. Statistical analysis using Kappa statistics showed that the degree of agreement was best seen between RBT and CFT (96%), followed by RBT, I- ELISA (91.4%) and CFT and I- ELISA (89.2%). Biochemical tests and species-specific Multiplex PCR showed the typical profile for B. melitensis. A structured questionnaire and Chi-square (x2) statistical analysis indicated that the presence of abortion is the major risk factor for the prevalence of brucellosis, whereas age and sex were not significant factors in the tested animals. Besides, poor knowledge about brucellosis, consumption of unpasteurized milk or milk products, free trade of animals and the introduction of new animal breeds to herds were all contributing risk factors to the prevalence of brucellosis. The prevalence of human brucellosis obtained verbally from pastoralists gave an insight that brucellosis could pose a public health hazard, especially in those high-risk groups, mainly the pastoralists in the study area. Because of their constant and increasing interaction with their animals, pastoralists could be at a high risk of occupational infection.

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