Role of the transcription factor T (brachyury) in the pathogenesis of sporadic chordoma: A genetic and functional-based study

Nadège Presneau, Asem Shalaby, Hongtao Ye, Nischalan Pillay, Dina Halai, Bernadine Idowu, Roberto Tirabosco, Duncan Whitwell, Thomas S. Jacques, Lars Gunnar Kindblom, Silke Brüderlein, Peter Möller, Andreas Leithner, Bernadette Liegl, Fernanda M. Amary, Nicholas N. Athanasou, Pancras Cw Hogendoorn, Fredrik Mertens, Karoly Szuhai, Adrienne M. Flanagan

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

A variety of analyses, including fluorescence in situ hybridization (FISH), quantitative PCR (qPCR) and array CGH (aCGH), have been performed on a series of chordomas from 181 patients. Twelve of 181 (7%) tumours displayed amplification of the T locus and an additional two cases showed focal amplification; 70/181 (39%) tumours were polysomic for chromosome 6, and 8/181 (4.5%) primary tumours showed a minor allelic gain of T as assessed by FISH. No germline alteration of the T locus was identified in non-neoplastic tissue from 40 patients. Copy number gain of T was seen in a similar percentage of sacrococcygeal, mobile spine and base of skull tumours. Knockdown of T in the cell line, U-CH1, which showed polysomy of chromosome 6 involving 6q27, resulted in a marked decrease in cell proliferation and morphological features consistent with a senescence-like phenotype. The U-CH1 cell line was validated as representing chordoma by the generation of xenografts, which showed typical chordoma morphology and immunohistochemistry in the NOD/SCID/interleukin 2 receptor [IL2r]γnull mouse model. In conclusion, chromosomal aberrations resulting in gain of the T locus are common in sporadic chordomas and expression of this gene is critical for proliferation of chordoma cells in vitro.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalJournal of Pathology
Volume223
Issue number3
DOIs
Publication statusPublished - Feb 2011

Keywords

  • Amplification
  • Chordoma
  • Copy number gain
  • Oncogene
  • T

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Presneau, N., Shalaby, A., Ye, H., Pillay, N., Halai, D., Idowu, B., Tirabosco, R., Whitwell, D., Jacques, T. S., Kindblom, L. G., Brüderlein, S., Möller, P., Leithner, A., Liegl, B., Amary, F. M., Athanasou, N. N., Hogendoorn, P. C., Mertens, F., Szuhai, K., & Flanagan, A. M. (2011). Role of the transcription factor T (brachyury) in the pathogenesis of sporadic chordoma: A genetic and functional-based study. Journal of Pathology, 223(3), 327-335. https://doi.org/10.1002/path.2816