Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of

Raeid M M Abed, Lubos Polerecky, Amal Al-Habsi, Janina Oetjen, Marc Strous, Dirk De Beer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top 〈1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2-0.4 mm and this layer did not move upward after wetting.13 C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92μmol m-2 h-1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting.

Original languageEnglish
Article numbere112372
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 6 2014

Fingerprint

soil crusts
Cyanobacteria
Pigments
Wetting
Microsensors
Soil
Color
pigments
Soils
chlorophyll
Recovery
Labeling
Water
color
image analysis
Desiccation
water
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
desorption
topsoil

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of. / Abed, Raeid M M; Polerecky, Lubos; Al-Habsi, Amal; Oetjen, Janina; Strous, Marc; De Beer, Dirk.

In: PLoS One, Vol. 9, No. 11, e112372, 06.11.2014.

Research output: Contribution to journalArticle

Abed, Raeid M M ; Polerecky, Lubos ; Al-Habsi, Amal ; Oetjen, Janina ; Strous, Marc ; De Beer, Dirk. / Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of. In: PLoS One. 2014 ; Vol. 9, No. 11.
@article{7b6c35756301400da07d019aaab34e98,
title = "Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of",
abstract = "We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top 〈1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2-0.4 mm and this layer did not move upward after wetting.13 C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92μmol m-2 h-1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting.",
author = "Abed, {Raeid M M} and Lubos Polerecky and Amal Al-Habsi and Janina Oetjen and Marc Strous and {De Beer}, Dirk",
year = "2014",
month = "11",
day = "6",
doi = "10.1371/journal.pone.0112372",
language = "English",
volume = "9",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Rapid recovery of cyanobacterial pigments in desiccated biological soil crusts following addition of

AU - Abed, Raeid M M

AU - Polerecky, Lubos

AU - Al-Habsi, Amal

AU - Oetjen, Janina

AU - Strous, Marc

AU - De Beer, Dirk

PY - 2014/11/6

Y1 - 2014/11/6

N2 - We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top 〈1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2-0.4 mm and this layer did not move upward after wetting.13 C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92μmol m-2 h-1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting.

AB - We examined soil surface colour change to green and hydrotaxis following addition of water to biological soil crusts using pigment extraction, hyperspectral imaging, microsensors and 13C labeling experiments coupled to matrix-assisted laser desorption and ionization time of flight-mass spectrometry (MALD-TOF MS). The topsoil colour turned green in less than 5 minutes following water addition. The concentrations of chlorophyll a (Chl a), scytonemin and echinenon rapidly increased in the top 〈1 mm layer while in the deeper layer, their concentrations remained low. Hyperspectral imaging showed that, in both wet and dehydrated crusts, cyanobacteria formed a layer at a depth of 0.2-0.4 mm and this layer did not move upward after wetting.13 C labeling experiments and MALDI TOF analysis showed that Chl a was already present in the desiccated crusts and de novo synthesis of this molecule started only after 2 days of wetting due to growth of cyanobacteria. Microsensor measurements showed that photosynthetic activity increased concomitantly with the increase of Chl a, and reached a maximum net rate of 92μmol m-2 h-1 approximately 2 hours after wetting. We conclude that the colour change of soil crusts to green upon water addition was not due to hydrotaxis but rather to the quick recovery and reassembly of pigments. Cyanobacteria in crusts can maintain their photosynthetic apparatus intact even under prolonged periods of desiccation with the ability to resume their photosynthetic activities within minutes after wetting.

UR - http://www.scopus.com/inward/record.url?scp=84910019789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910019789&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0112372

DO - 10.1371/journal.pone.0112372

M3 - Article

C2 - 25375172

AN - SCOPUS:84910019789

VL - 9

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e112372

ER -