TY - JOUR
T1 - Quantification of busulfan in plasma by gas chromatography-mass spectrometry following derivatization with tetrafluorothiophenol
AU - Quernin, Marie Helene
AU - Poonkuzhali, Balasubramanian
AU - Montes, Christine
AU - Krishnamoorthy, Rajagopal
AU - Dennison, David
AU - Srivastava, Alok
AU - Vilmer, Etienne
AU - Chandy, Mammen
AU - Jacqz-Aigrain, Evelyne
N1 - Funding Information:
We are grateful to Gilles Vassal, Clinical Pharmacology Unit (UA147 CNRS, U140 INSERM), Institut Gustave-Roussy, 94805 Villejuif, France, for analysing the samples with the GC–MS method using sodium iodide derivatization. This Indo-French network was made possible by the financial assistance from Inserm, France, under the grant No. 94NS4 attributed to Dr. R. Krishnamoorthy and the Indian Council of Medical Research for the “Advanced Center for Bone Marrow Transplantation for Thalassaemia in India” at Christian Medical College and Hospital, Vellore, India.
PY - 1998/5/8
Y1 - 1998/5/8
N2 - A specific and highly sensitive method has been developed for the determination of busulfan in plasma by gas chromatography-mass spectrometry using a deuterium-labeled busulfan (busulfan-d8) as internal standard. Plasma containing busulfan and busulfan-d8 were extracted with ethyl acetate and derivatized with 2,3,5,6-tetrafluorothiophenol prior to the monitoring of specific ions. The limit of quantification of the assay was 20 ng/ml and the calibration curve was linear over the range of 10 to 2000 ng/ml of derivatized busulfan. This method was in good agreement with the GC-MS assay using derivatization with sodium iodide and measuring diiodobutane. In addition, a pharmacokinetic study of busulfan was conducted in six children. The apparent oral clearance was 5.7 ± 1.9 ml/kg/min and the volume of distribution was 1.0 ± 0.4 l/kg and were similar to those previously reported in pediatric patients.
AB - A specific and highly sensitive method has been developed for the determination of busulfan in plasma by gas chromatography-mass spectrometry using a deuterium-labeled busulfan (busulfan-d8) as internal standard. Plasma containing busulfan and busulfan-d8 were extracted with ethyl acetate and derivatized with 2,3,5,6-tetrafluorothiophenol prior to the monitoring of specific ions. The limit of quantification of the assay was 20 ng/ml and the calibration curve was linear over the range of 10 to 2000 ng/ml of derivatized busulfan. This method was in good agreement with the GC-MS assay using derivatization with sodium iodide and measuring diiodobutane. In addition, a pharmacokinetic study of busulfan was conducted in six children. The apparent oral clearance was 5.7 ± 1.9 ml/kg/min and the volume of distribution was 1.0 ± 0.4 l/kg and were similar to those previously reported in pediatric patients.
KW - Busulfan
KW - Tetrafluorothiophenol
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U2 - 10.1016/S0378-4347(98)00019-X
DO - 10.1016/S0378-4347(98)00019-X
M3 - Article
C2 - 9653925
AN - SCOPUS:0032496254
SN - 1572-6495
VL - 709
SP - 47
EP - 56
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 1
ER -