Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells

R. Velazhahan, R. Samiyappan, P. Vidhyasekaran

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured cells were treated with an elicitor isolated from Rhizoctonia solani, the rice sheath blight pathogen. A fivefold increase in chitinase activity was observed 24 h after elicitor treatment. The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa and it has an isoelectric point of 8.3. The 35 kDa basic chitinase inhibited mycelial growth of R. solani in vitro. Morphological changes appeared within 1 h following exposure of mycelium to the chitinase. The hyphal tip showed marked swelling and subsequently lysis was also observed.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalPhytoparasitica
Volume28
Issue number2
Publication statusPublished - 2000

Fingerprint

chitinase
rice
Thanatephorus cucumeris
cells
affinity chromatography
isoelectric point
chitin
blight
ammonium sulfate
cultured cells
mycelium
fractionation
elicitors
gels
molecular weight
pathogens

Keywords

  • Basic chitinase
  • Hyphal lysis
  • Oryza sativa
  • Rhizoctonia solani
  • Sheath blight

ASJC Scopus subject areas

  • Plant Science
  • Insect Science

Cite this

Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells. / Velazhahan, R.; Samiyappan, R.; Vidhyasekaran, P.

In: Phytoparasitica, Vol. 28, No. 2, 2000, p. 131-139.

Research output: Contribution to journalArticle

Velazhahan, R. ; Samiyappan, R. ; Vidhyasekaran, P. / Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells. In: Phytoparasitica. 2000 ; Vol. 28, No. 2. pp. 131-139.
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