Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells

R. Velazhahan; R. Samiyappan; P. Vidhyasekaran

doi:10.1007/BF02981742

Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells

R. Velazhahan^*, R. Samiyappan, P. Vidhyasekaran

^*Corresponding author for this work

Crop Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured cells were treated with an elicitor isolated from Rhizoctonia solani, the rice sheath blight pathogen. A fivefold increase in chitinase activity was observed 24 h after elicitor treatment. The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa and it has an isoelectric point of 8.3. The 35 kDa basic chitinase inhibited mycelial growth of R. solani in vitro. Morphological changes appeared within 1 h following exposure of mycelium to the chitinase. The hyphal tip showed marked swelling and subsequently lysis was also observed.

Original language	English
Pages (from-to)	131-139
Number of pages	9
Journal	Phytoparasitica
Volume	28
Issue number	2
DOIs	https://doi.org/10.1007/BF02981742
Publication status	Published - 2000

Keywords

Basic chitinase
Hyphal lysis
Oryza sativa
Rhizoctonia solani
Sheath blight

ASJC Scopus subject areas

Plant Science
Insect Science

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10.1007/BF02981742

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@article{9053cb6ded294bb8931c59068a1c740d,

title = "Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells",

abstract = "Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured cells were treated with an elicitor isolated from Rhizoctonia solani, the rice sheath blight pathogen. A fivefold increase in chitinase activity was observed 24 h after elicitor treatment. The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa and it has an isoelectric point of 8.3. The 35 kDa basic chitinase inhibited mycelial growth of R. solani in vitro. Morphological changes appeared within 1 h following exposure of mycelium to the chitinase. The hyphal tip showed marked swelling and subsequently lysis was also observed.",

keywords = "Basic chitinase, Hyphal lysis, Oryza sativa, Rhizoctonia solani, Sheath blight",

author = "R. Velazhahan and R. Samiyappan and P. Vidhyasekaran",

note = "Funding Information: This work was supported by the Rockefeller Foundation, USA. We are grateful to Dr. S. Muthukrishnan, Kansas State University, Manhattan, KS, USA, tbr the gift of chitinase antiserum.",

year = "2000",

doi = "10.1007/BF02981742",

language = "English",

volume = "28",

pages = "131--139",

journal = "Phytoparasitica",

issn = "0334-2123",

publisher = "Springer Netherlands",

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TY - JOUR

T1 - Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells

AU - Velazhahan, R.

AU - Samiyappan, R.

AU - Vidhyasekaran, P.

N1 - Funding Information: This work was supported by the Rockefeller Foundation, USA. We are grateful to Dr. S. Muthukrishnan, Kansas State University, Manhattan, KS, USA, tbr the gift of chitinase antiserum.

PY - 2000

Y1 - 2000

N2 - Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured cells were treated with an elicitor isolated from Rhizoctonia solani, the rice sheath blight pathogen. A fivefold increase in chitinase activity was observed 24 h after elicitor treatment. The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa and it has an isoelectric point of 8.3. The 35 kDa basic chitinase inhibited mycelial growth of R. solani in vitro. Morphological changes appeared within 1 h following exposure of mycelium to the chitinase. The hyphal tip showed marked swelling and subsequently lysis was also observed.

AB - Suspension-cultured rice cells showed an appreciable amount of chitinase activity when the cultured cells were treated with an elicitor isolated from Rhizoctonia solani, the rice sheath blight pathogen. A fivefold increase in chitinase activity was observed 24 h after elicitor treatment. The elicitor-inducible chitinase was purified by ammonium sulfate fractionation, chitin affinity chromatography and gel filtration. Its molecular weight is 35 kDa and it has an isoelectric point of 8.3. The 35 kDa basic chitinase inhibited mycelial growth of R. solani in vitro. Morphological changes appeared within 1 h following exposure of mycelium to the chitinase. The hyphal tip showed marked swelling and subsequently lysis was also observed.

KW - Basic chitinase

KW - Hyphal lysis

KW - Oryza sativa

KW - Rhizoctonia solani

KW - Sheath blight

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U2 - 10.1007/BF02981742

DO - 10.1007/BF02981742

M3 - Article

AN - SCOPUS:0034081903

SN - 0334-2123

VL - 28

SP - 131

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JO - Phytoparasitica

JF - Phytoparasitica

IS - 2

ER -

Purification of an elicitor-inducible antifungal chitinase from suspension-cultured rice cells

Abstract

Keywords

ASJC Scopus subject areas

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