Purification and partial characterization of a toxin produced by Ganoderma lucidum, the coconut Ganoderma disease pathogen

M. Karthikeyan, R. Bhaskaran, K. Radhika, S. Mathiyazhagan, R. Sandosskumar, R. Samiyappan, R. Velazhahan

Research output: Contribution to journalArticle

Abstract

An extracellular, hydrophilic, thermostable phytotoxin was purified to homogeneity from culture fluids of Ganoderma lucidum. The phytotoxin was purified by solvent extraction, gel filtration on Sephadex G-75. Toxicity was evaluated with detached leaf sheath and electrolyte leakage bioassays. Purified phytotoxin induced visible symptoms of the disease, when applied to coconut leaves, fronds and roots even at a low concentration. The toxin is a glycoprotein with carbohydrate as the major component. The importance of the carbohydrate moiety for toxic activity was indicated by inactivation of toxic compounds after periodate oxidation. The toxin caused lesions on a number of other monocots and dicots and proved to be non-host specific.

Original languageEnglish
Pages (from-to)507-513
Number of pages7
JournalArchives of Phytopathology and Plant Protection
Volume41
Issue number7
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Ganoderma
Ganoderma lucidum
phytotoxins
coconuts
toxins
pathogens
carbohydrates
lesions (plant)
fronds
Magnoliopsida
Liliopsida
toxic substances
signs and symptoms (plants)
electrolytes
glycoproteins
leaves
inactivation
bioassays
gels
oxidation

Keywords

  • Carbohydrate
  • Coconut
  • Ganoderma lucidum
  • Phytotoxin
  • Protein

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Purification and partial characterization of a toxin produced by Ganoderma lucidum, the coconut Ganoderma disease pathogen. / Karthikeyan, M.; Bhaskaran, R.; Radhika, K.; Mathiyazhagan, S.; Sandosskumar, R.; Samiyappan, R.; Velazhahan, R.

In: Archives of Phytopathology and Plant Protection, Vol. 41, No. 7, 10.2008, p. 507-513.

Research output: Contribution to journalArticle

Karthikeyan, M. ; Bhaskaran, R. ; Radhika, K. ; Mathiyazhagan, S. ; Sandosskumar, R. ; Samiyappan, R. ; Velazhahan, R. / Purification and partial characterization of a toxin produced by Ganoderma lucidum, the coconut Ganoderma disease pathogen. In: Archives of Phytopathology and Plant Protection. 2008 ; Vol. 41, No. 7. pp. 507-513.
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