Purification and characterization of an antifungal thaumatin-like protein from sorghum (Sorghum bicolor) leaves

R. Velazhahan, J. Jayaraj, J. M. Jeoung, G. H. Liang, S. Muthukrishnan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A protein with an apparent molecular mass of 17 kDa that cross-reacts with an antibody to corn zeamatin was induced in sorghum (Sorghum bicolor (L.) Moench) plants when infected with Fusarium moniliforme, the causal agent of stalk rot. The 17-kDa protein was purified from F. moniliforme-inoculated sorghum tissues by ammonium sulfate fractionation, anion exchange chromatography on DEAE-Sephacel followed by gel filtration on a Sephadex G-75 column. The N-terminal amino acid sequence analysis of the purified 17-kDa protein revealed sequence homology to thaumatin-like proteins (TLPs) of maize, barley, wheat, oats, rice and zeamatin. Western blot analysis showed that the purified 17-kDa TLP cross-reacted well with zeamatin antiserum and to a lesser extent with a bean TLP antiserum. The purified 17-kDa TLP inhibited the mycelial growth of F. moniliforme and Trichoderma viride in vitro.

Original languageEnglish
Pages (from-to)452-461
Number of pages10
JournalZeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Volume109
Issue number5
Publication statusPublished - Sep 2002

Fingerprint

Sorghum bicolor
Sorghum (Poaceae)
leaves
proteins
antiserum
amino acid sequences
Trichoderma viride
corn
sequence homology
ammonium sulfate
oats
fractionation
beans
Western blotting
sequence analysis
barley
gels
molecular weight
rice
wheat

Keywords

  • Amino acid sequence
  • Fusarium moniliforme
  • PR-5 protein
  • Sorghum bicolor
  • Trichoderma viride

ASJC Scopus subject areas

  • Plant Science

Cite this

Purification and characterization of an antifungal thaumatin-like protein from sorghum (Sorghum bicolor) leaves. / Velazhahan, R.; Jayaraj, J.; Jeoung, J. M.; Liang, G. H.; Muthukrishnan, S.

In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, Vol. 109, No. 5, 09.2002, p. 452-461.

Research output: Contribution to journalArticle

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