TY - JOUR
T1 - Protective effect of zizyphus spinachristi on mpp+-induced oxidative stress
AU - Singh, Vandita
AU - Essa, Musthafa Mohamed
AU - Guizani, Nejib
AU - Balakrishnan, Rengasamy
AU - Hemalatha, Tamiloli
AU - Manivasagam, Thamilarasan
AU - Justin-Thenmozhi, Arokiasamy
AU - Elangovan, Namasivayam
AU - Velusamy, Thirunavukkarasu
N1 - Publisher Copyright:
© 1996-2018
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Oxidative stress and mitochondrial dysfunction mediated neuro apoptosis is reported to play a major role in the pathology of Parkinson’s disease. Zizyphus spina-christi fruits (ZSCF) are used as traditional medicines that are well-known for their high antioxidant properties. In the present study, we investigated the protective effects of ZSCF extract against 1-methyl-4-phenylpyridinium (MPP+) induced neurotoxicity in SH-SY5Y cell lines. The effect of ZCSF on MPP+ induced cell viability (MTT - 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay), membrane damage - (lactate dehydrogenase (LDH), oxidative stress (levels of ROS, nitric oxide and GSH and activities of SOD and catalase), mitochondrial membrane potential and apoptosis (activity of caspase 3 and protein expressions of cyto c, Bax and Bcl-2) were measured. Our results showed that ZSCF could be able to reduce the neurotoxicity of MPP+ and offer neuroprotection in vitro. This protective effect of ZCF might be mediated by its potent antioxidant properties. However, further research is necessary to isolate active compounds and performing preclinical and clinical studies to confirm the neuro-protective effects of ZSCF in PD.
AB - Oxidative stress and mitochondrial dysfunction mediated neuro apoptosis is reported to play a major role in the pathology of Parkinson’s disease. Zizyphus spina-christi fruits (ZSCF) are used as traditional medicines that are well-known for their high antioxidant properties. In the present study, we investigated the protective effects of ZSCF extract against 1-methyl-4-phenylpyridinium (MPP+) induced neurotoxicity in SH-SY5Y cell lines. The effect of ZCSF on MPP+ induced cell viability (MTT - 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay), membrane damage - (lactate dehydrogenase (LDH), oxidative stress (levels of ROS, nitric oxide and GSH and activities of SOD and catalase), mitochondrial membrane potential and apoptosis (activity of caspase 3 and protein expressions of cyto c, Bax and Bcl-2) were measured. Our results showed that ZSCF could be able to reduce the neurotoxicity of MPP+ and offer neuroprotection in vitro. This protective effect of ZCF might be mediated by its potent antioxidant properties. However, further research is necessary to isolate active compounds and performing preclinical and clinical studies to confirm the neuro-protective effects of ZSCF in PD.
KW - Apoptosis
KW - Mitochondrial dysfunction
KW - Oxidative Stress
KW - Parkinson’s disease
KW - SH-SY5Y cells
KW - Zizyphusspina Christi Fruit Extract
UR - http://www.scopus.com/inward/record.url?scp=85047333020&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047333020&partnerID=8YFLogxK
M3 - Article
C2 - 29293433
AN - SCOPUS:85047333020
SN - 1945-0516
VL - 10
SP - 285
EP - 299
JO - Frontiers in Bioscience - Scholar
JF - Frontiers in Bioscience - Scholar
IS - 2
ER -