Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman

Abdullah M. Al-Sadi, Ibtihal J. Al-Raisi, Masood Al-Azri, Hamoud Al-Hasani, Mohammed S. Al-Shukaili, Saif M. Al-Shuraiqi, Khater O. Al-Fahdi, Mike L. Deadman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In 2004, severe powdery mildew infection on peach occurred in Al-Jabal Al-Akdhar, Oman, and resulted in substantial yield losses to growers. This study was conducted to investigate occurrence, causal agents, genetic diversity and efficacy of azoxystrobin in management of this disease. Powdery mildew was observed on all farms and peach trees in Al-Jabal Al-Akdhar. Disease symptoms were first observed on shoots in April, followed by appearance on fruits. Disease severity reached its peak between May and June. Morphological and molecular identification of 22 powdery mildew isolates indicated that all belong to Podosphaera pannosa. Podosphaera pannosa reproduced the same symptoms upon inoculation on peach leaves. Amplified fragment length polymorphisms analysis of 35 isolates of P. pannosa from five different villages using four primer pair combinations produced 688 polymorphic loci and 35 different genotypes. Populations of P. pannosa were found to have low levels of gene diversity (H = 0.1858), which suggests that P. pannosa has been recently introduced into Al-Jabal Al-Akdhar. Analysis of molecular variance showed low levels of genetic differentiation among populations from the different villages, implying the introduction of P. pannosa into the different villages via common sources as well as frequent movement of pathogen inoculum among the different villages. Evaluating the efficacy of azoxystrobin showed that azoxystrobin is as efficacious as thiophanate-methyl in managing the disease, with sulphur being the least efficacious. The study is the first to report the presence of P. pannosa in Oman. Also reported are its genetic diversity and its management under commercial conditions.

Original languageEnglish
Pages (from-to)647-654
Number of pages8
JournalJournal of Phytopathology
Volume160
Issue number11-12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Oman
Podosphaera
powdery mildew
peaches
villages
population structure
Thiophanate
Amplified Fragment Length Polymorphism Analysis
genetic variation
signs and symptoms (plants)
Population
Disease Management
thiophanate-methyl
Sulfur
Fruit
Analysis of Variance
Genotype
disease severity
amplified fragment length polymorphism
growers

Keywords

  • ITS rDNA
  • Ortiva
  • Rose
  • Sphaerotheca pannosa
  • Topsin

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Physiology
  • Genetics

Cite this

Al-Sadi, A. M., Al-Raisi, I. J., Al-Azri, M., Al-Hasani, H., Al-Shukaili, M. S., Al-Shuraiqi, S. M., ... Deadman, M. L. (2012). Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman. Journal of Phytopathology, 160(11-12), 647-654. https://doi.org/10.1111/j.1439-0434.2012.01955.x

Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman. / Al-Sadi, Abdullah M.; Al-Raisi, Ibtihal J.; Al-Azri, Masood; Al-Hasani, Hamoud; Al-Shukaili, Mohammed S.; Al-Shuraiqi, Saif M.; Al-Fahdi, Khater O.; Deadman, Mike L.

In: Journal of Phytopathology, Vol. 160, No. 11-12, 12.2012, p. 647-654.

Research output: Contribution to journalArticle

Al-Sadi, AM, Al-Raisi, IJ, Al-Azri, M, Al-Hasani, H, Al-Shukaili, MS, Al-Shuraiqi, SM, Al-Fahdi, KO & Deadman, ML 2012, 'Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman', Journal of Phytopathology, vol. 160, no. 11-12, pp. 647-654. https://doi.org/10.1111/j.1439-0434.2012.01955.x
Al-Sadi, Abdullah M. ; Al-Raisi, Ibtihal J. ; Al-Azri, Masood ; Al-Hasani, Hamoud ; Al-Shukaili, Mohammed S. ; Al-Shuraiqi, Saif M. ; Al-Fahdi, Khater O. ; Deadman, Mike L. / Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman. In: Journal of Phytopathology. 2012 ; Vol. 160, No. 11-12. pp. 647-654.
@article{8384cf1d657d4423a7921ff6630fe7dc,
title = "Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman",
abstract = "In 2004, severe powdery mildew infection on peach occurred in Al-Jabal Al-Akdhar, Oman, and resulted in substantial yield losses to growers. This study was conducted to investigate occurrence, causal agents, genetic diversity and efficacy of azoxystrobin in management of this disease. Powdery mildew was observed on all farms and peach trees in Al-Jabal Al-Akdhar. Disease symptoms were first observed on shoots in April, followed by appearance on fruits. Disease severity reached its peak between May and June. Morphological and molecular identification of 22 powdery mildew isolates indicated that all belong to Podosphaera pannosa. Podosphaera pannosa reproduced the same symptoms upon inoculation on peach leaves. Amplified fragment length polymorphisms analysis of 35 isolates of P. pannosa from five different villages using four primer pair combinations produced 688 polymorphic loci and 35 different genotypes. Populations of P. pannosa were found to have low levels of gene diversity (H = 0.1858), which suggests that P. pannosa has been recently introduced into Al-Jabal Al-Akdhar. Analysis of molecular variance showed low levels of genetic differentiation among populations from the different villages, implying the introduction of P. pannosa into the different villages via common sources as well as frequent movement of pathogen inoculum among the different villages. Evaluating the efficacy of azoxystrobin showed that azoxystrobin is as efficacious as thiophanate-methyl in managing the disease, with sulphur being the least efficacious. The study is the first to report the presence of P. pannosa in Oman. Also reported are its genetic diversity and its management under commercial conditions.",
keywords = "ITS rDNA, Ortiva, Rose, Sphaerotheca pannosa, Topsin",
author = "Al-Sadi, {Abdullah M.} and Al-Raisi, {Ibtihal J.} and Masood Al-Azri and Hamoud Al-Hasani and Al-Shukaili, {Mohammed S.} and Al-Shuraiqi, {Saif M.} and Al-Fahdi, {Khater O.} and Deadman, {Mike L.}",
year = "2012",
month = "12",
doi = "10.1111/j.1439-0434.2012.01955.x",
language = "English",
volume = "160",
pages = "647--654",
journal = "Journal of Phytopathology",
issn = "0931-1785",
publisher = "Wiley-Blackwell",
number = "11-12",

}

TY - JOUR

T1 - Population Structure and Management of Podosphaera pannosa Associated with Peach Powdery Mildew in Oman

AU - Al-Sadi, Abdullah M.

AU - Al-Raisi, Ibtihal J.

AU - Al-Azri, Masood

AU - Al-Hasani, Hamoud

AU - Al-Shukaili, Mohammed S.

AU - Al-Shuraiqi, Saif M.

AU - Al-Fahdi, Khater O.

AU - Deadman, Mike L.

PY - 2012/12

Y1 - 2012/12

N2 - In 2004, severe powdery mildew infection on peach occurred in Al-Jabal Al-Akdhar, Oman, and resulted in substantial yield losses to growers. This study was conducted to investigate occurrence, causal agents, genetic diversity and efficacy of azoxystrobin in management of this disease. Powdery mildew was observed on all farms and peach trees in Al-Jabal Al-Akdhar. Disease symptoms were first observed on shoots in April, followed by appearance on fruits. Disease severity reached its peak between May and June. Morphological and molecular identification of 22 powdery mildew isolates indicated that all belong to Podosphaera pannosa. Podosphaera pannosa reproduced the same symptoms upon inoculation on peach leaves. Amplified fragment length polymorphisms analysis of 35 isolates of P. pannosa from five different villages using four primer pair combinations produced 688 polymorphic loci and 35 different genotypes. Populations of P. pannosa were found to have low levels of gene diversity (H = 0.1858), which suggests that P. pannosa has been recently introduced into Al-Jabal Al-Akdhar. Analysis of molecular variance showed low levels of genetic differentiation among populations from the different villages, implying the introduction of P. pannosa into the different villages via common sources as well as frequent movement of pathogen inoculum among the different villages. Evaluating the efficacy of azoxystrobin showed that azoxystrobin is as efficacious as thiophanate-methyl in managing the disease, with sulphur being the least efficacious. The study is the first to report the presence of P. pannosa in Oman. Also reported are its genetic diversity and its management under commercial conditions.

AB - In 2004, severe powdery mildew infection on peach occurred in Al-Jabal Al-Akdhar, Oman, and resulted in substantial yield losses to growers. This study was conducted to investigate occurrence, causal agents, genetic diversity and efficacy of azoxystrobin in management of this disease. Powdery mildew was observed on all farms and peach trees in Al-Jabal Al-Akdhar. Disease symptoms were first observed on shoots in April, followed by appearance on fruits. Disease severity reached its peak between May and June. Morphological and molecular identification of 22 powdery mildew isolates indicated that all belong to Podosphaera pannosa. Podosphaera pannosa reproduced the same symptoms upon inoculation on peach leaves. Amplified fragment length polymorphisms analysis of 35 isolates of P. pannosa from five different villages using four primer pair combinations produced 688 polymorphic loci and 35 different genotypes. Populations of P. pannosa were found to have low levels of gene diversity (H = 0.1858), which suggests that P. pannosa has been recently introduced into Al-Jabal Al-Akdhar. Analysis of molecular variance showed low levels of genetic differentiation among populations from the different villages, implying the introduction of P. pannosa into the different villages via common sources as well as frequent movement of pathogen inoculum among the different villages. Evaluating the efficacy of azoxystrobin showed that azoxystrobin is as efficacious as thiophanate-methyl in managing the disease, with sulphur being the least efficacious. The study is the first to report the presence of P. pannosa in Oman. Also reported are its genetic diversity and its management under commercial conditions.

KW - ITS rDNA

KW - Ortiva

KW - Rose

KW - Sphaerotheca pannosa

KW - Topsin

UR - http://www.scopus.com/inward/record.url?scp=84868197746&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868197746&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0434.2012.01955.x

DO - 10.1111/j.1439-0434.2012.01955.x

M3 - Article

VL - 160

SP - 647

EP - 654

JO - Journal of Phytopathology

JF - Journal of Phytopathology

SN - 0931-1785

IS - 11-12

ER -