Abstract
Plants and their by-products offer a diverse mixture of chemical constituents like natural antioxidants. Datepits are rich in phenolic compounds that have antioxidant potential. The main objective of this study was to investigate the protective effect of a date-pit extract (DPE) against AOM-induced colonic carcinogenicity and oxidative stress. Thirty-two weanling male Sprauge-Dawley rats were randomly divided into four groups (eight rats in each group). All rats were fed basic diet and water ad libitum, and randomly distributed per treatment groups as follows: negative controls injected with normal saline once a week for two weeks, a cancer group injected intra-peritoneally with azoxymethane (15mg/kg body weight) for two consecutive weeks, and DPE treated groups receiving the extract via the oral route (1.5ml/day) for the entire experiment in the presence or absence of AOM injection. Results showed that DPE contained phytonutrients that were capable of inhibiting chemically-induced oxidative stress in the rat colonic cells. In those animals that consumed DPE, a protective effect was observed against AOM-induced oxidative stress in rat colonic cells as evident by a significant decrease in MDA and oxidized DCF formation in AOM injected and DPE fed groups. It is concluded that DPE has potential antioxidant and anticarcinogenic properties.
| Original language | English |
|---|---|
| Pages (from-to) | 3473-3477 |
| Number of pages | 5 |
| Journal | Asian Pacific Journal of Cancer Prevention |
| Volume | 16 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Azoxymethane
- Colon cancer
- Date pit extract
- Oxidative stress
- Total antioxidant capacity
ASJC Scopus subject areas
- Oncology
- Cancer Research
- Public Health, Environmental and Occupational Health
- Epidemiology
Cite this
Phytonutrient effects of date pit extract against azoxymethane-induced oxidative stress in the rat colon. / Waly, Mostafa Ibrahim; Al-Ghafri, Bushra Rashid; Guizani, Nejib; Rahman, Mohammad Shafiur.
In: Asian Pacific Journal of Cancer Prevention, Vol. 16, No. 8, 2015, p. 3473-3477.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phytonutrient effects of date pit extract against azoxymethane-induced oxidative stress in the rat colon
AU - Waly, Mostafa Ibrahim
AU - Al-Ghafri, Bushra Rashid
AU - Guizani, Nejib
AU - Rahman, Mohammad Shafiur
PY - 2015
Y1 - 2015
N2 - Plants and their by-products offer a diverse mixture of chemical constituents like natural antioxidants. Datepits are rich in phenolic compounds that have antioxidant potential. The main objective of this study was to investigate the protective effect of a date-pit extract (DPE) against AOM-induced colonic carcinogenicity and oxidative stress. Thirty-two weanling male Sprauge-Dawley rats were randomly divided into four groups (eight rats in each group). All rats were fed basic diet and water ad libitum, and randomly distributed per treatment groups as follows: negative controls injected with normal saline once a week for two weeks, a cancer group injected intra-peritoneally with azoxymethane (15mg/kg body weight) for two consecutive weeks, and DPE treated groups receiving the extract via the oral route (1.5ml/day) for the entire experiment in the presence or absence of AOM injection. Results showed that DPE contained phytonutrients that were capable of inhibiting chemically-induced oxidative stress in the rat colonic cells. In those animals that consumed DPE, a protective effect was observed against AOM-induced oxidative stress in rat colonic cells as evident by a significant decrease in MDA and oxidized DCF formation in AOM injected and DPE fed groups. It is concluded that DPE has potential antioxidant and anticarcinogenic properties.
AB - Plants and their by-products offer a diverse mixture of chemical constituents like natural antioxidants. Datepits are rich in phenolic compounds that have antioxidant potential. The main objective of this study was to investigate the protective effect of a date-pit extract (DPE) against AOM-induced colonic carcinogenicity and oxidative stress. Thirty-two weanling male Sprauge-Dawley rats were randomly divided into four groups (eight rats in each group). All rats were fed basic diet and water ad libitum, and randomly distributed per treatment groups as follows: negative controls injected with normal saline once a week for two weeks, a cancer group injected intra-peritoneally with azoxymethane (15mg/kg body weight) for two consecutive weeks, and DPE treated groups receiving the extract via the oral route (1.5ml/day) for the entire experiment in the presence or absence of AOM injection. Results showed that DPE contained phytonutrients that were capable of inhibiting chemically-induced oxidative stress in the rat colonic cells. In those animals that consumed DPE, a protective effect was observed against AOM-induced oxidative stress in rat colonic cells as evident by a significant decrease in MDA and oxidized DCF formation in AOM injected and DPE fed groups. It is concluded that DPE has potential antioxidant and anticarcinogenic properties.
KW - Azoxymethane
KW - Colon cancer
KW - Date pit extract
KW - Oxidative stress
KW - Total antioxidant capacity
UR - http://www.scopus.com/inward/record.url?scp=84929012834&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929012834&partnerID=8YFLogxK
U2 - 10.7314/APJCP.2015.16.8.3473
DO - 10.7314/APJCP.2015.16.8.3473
M3 - Article
VL - 16
SP - 3473
EP - 3477
JO - Asian Pacific Journal of Cancer Prevention
JF - Asian Pacific Journal of Cancer Prevention
SN - 1513-7368
IS - 8
ER -