Photoinduced oxidation of a tris(2,2'-bipyridyl)ruthenium(II)- peroxodisulfate chemiluminescence system for the analysis of mebeverine HCl pharmaceutical formulations and biological fluids using a two-chip device

Haider A J Al Lawati, Zeiyana M. Al Dahmani, Gouri B. Varma, Fakhreldin O. Suliman

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Abstract

A new method for the analysis of mebeverine hydrochloride (MEB) has been developed using a two-chip device. The method is highly selective, sensitive, rapid and consumes minute amount of reagents. The developed method is free of interference from the degradation products of MEB and from common ingredients present in pharmaceutical formulations. The limit of detection was 0.043 μg/mL, and the limit of quantification was 0.138 μg/mL. The short analysis time per sample (20 s) allowed a large number of analyses to be performed within a very short time. Various samples were analyzed, including two different pharmaceutical formulations and a uniformity of content analysis for 20 tablets from a known batch and two biological samples at different concentrations. In addition, the method was compared with a validated high-performance liquid chromatography (HPLC) method and the results clearly indicated the suitability of the developed method for routine analyses. A new mechanism for the tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3 2+)- peroxodisulfate (S2O8 2-) chemiluminescence (CL) system has also been proposed. The mechanism is based on photoinduced oxidation of Ru(bpy)3 2+ to Ru(bpy)3 3+ via the formation of Ru(bpy)3 2+* upon irradiation with visible light. S2O8 2- then oxidizes Ru(bpy)3 2+* to Ru(bpy)3 3+ and the analyte subsequently reduces the resultant Ru(bpy)3 3+ to Ru(bpy)3 2+*, which then produces the CL signal.

Original languageEnglish
Pages (from-to)275-283
Number of pages9
JournalLuminescence
Volume29
Issue number3
DOIs
Publication statusPublished - 2014

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Drug Compounding
Chemiluminescence
Systems Analysis
Luminescence
Equipment and Supplies
Oxidation
Fluids
High performance liquid chromatography
Pharmaceutical Preparations
Tablets
Irradiation
Degradation
Limit of Detection
tris(2,2'-bipyridyl)ruthenium(II)
mebeverine
High Pressure Liquid Chromatography
Light

Keywords

  • chemiluminscence
  • mebeverine
  • microfluidics
  • photoinduced oxidation
  • tris(2,2-bipyridyl)ruthenium(II)

ASJC Scopus subject areas

  • Biophysics
  • Chemistry (miscellaneous)
  • Medicine(all)

Cite this

@article{e120cbfd2c514b3ea8fa4f90c1116b05,
title = "Photoinduced oxidation of a tris(2,2'-bipyridyl)ruthenium(II)- peroxodisulfate chemiluminescence system for the analysis of mebeverine HCl pharmaceutical formulations and biological fluids using a two-chip device",
abstract = "A new method for the analysis of mebeverine hydrochloride (MEB) has been developed using a two-chip device. The method is highly selective, sensitive, rapid and consumes minute amount of reagents. The developed method is free of interference from the degradation products of MEB and from common ingredients present in pharmaceutical formulations. The limit of detection was 0.043 μg/mL, and the limit of quantification was 0.138 μg/mL. The short analysis time per sample (20 s) allowed a large number of analyses to be performed within a very short time. Various samples were analyzed, including two different pharmaceutical formulations and a uniformity of content analysis for 20 tablets from a known batch and two biological samples at different concentrations. In addition, the method was compared with a validated high-performance liquid chromatography (HPLC) method and the results clearly indicated the suitability of the developed method for routine analyses. A new mechanism for the tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3 2+)- peroxodisulfate (S2O8 2-) chemiluminescence (CL) system has also been proposed. The mechanism is based on photoinduced oxidation of Ru(bpy)3 2+ to Ru(bpy)3 3+ via the formation of Ru(bpy)3 2+* upon irradiation with visible light. S2O8 2- then oxidizes Ru(bpy)3 2+* to Ru(bpy)3 3+ and the analyte subsequently reduces the resultant Ru(bpy)3 3+ to Ru(bpy)3 2+*, which then produces the CL signal.",
keywords = "chemiluminscence, mebeverine, microfluidics, photoinduced oxidation, tris(2,2-bipyridyl)ruthenium(II)",
author = "{Al Lawati}, {Haider A J} and {Al Dahmani}, {Zeiyana M.} and Varma, {Gouri B.} and Suliman, {Fakhreldin O.}",
year = "2014",
doi = "10.1002/bio.2540",
language = "English",
volume = "29",
pages = "275--283",
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TY - JOUR

T1 - Photoinduced oxidation of a tris(2,2'-bipyridyl)ruthenium(II)- peroxodisulfate chemiluminescence system for the analysis of mebeverine HCl pharmaceutical formulations and biological fluids using a two-chip device

AU - Al Lawati, Haider A J

AU - Al Dahmani, Zeiyana M.

AU - Varma, Gouri B.

AU - Suliman, Fakhreldin O.

PY - 2014

Y1 - 2014

N2 - A new method for the analysis of mebeverine hydrochloride (MEB) has been developed using a two-chip device. The method is highly selective, sensitive, rapid and consumes minute amount of reagents. The developed method is free of interference from the degradation products of MEB and from common ingredients present in pharmaceutical formulations. The limit of detection was 0.043 μg/mL, and the limit of quantification was 0.138 μg/mL. The short analysis time per sample (20 s) allowed a large number of analyses to be performed within a very short time. Various samples were analyzed, including two different pharmaceutical formulations and a uniformity of content analysis for 20 tablets from a known batch and two biological samples at different concentrations. In addition, the method was compared with a validated high-performance liquid chromatography (HPLC) method and the results clearly indicated the suitability of the developed method for routine analyses. A new mechanism for the tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3 2+)- peroxodisulfate (S2O8 2-) chemiluminescence (CL) system has also been proposed. The mechanism is based on photoinduced oxidation of Ru(bpy)3 2+ to Ru(bpy)3 3+ via the formation of Ru(bpy)3 2+* upon irradiation with visible light. S2O8 2- then oxidizes Ru(bpy)3 2+* to Ru(bpy)3 3+ and the analyte subsequently reduces the resultant Ru(bpy)3 3+ to Ru(bpy)3 2+*, which then produces the CL signal.

AB - A new method for the analysis of mebeverine hydrochloride (MEB) has been developed using a two-chip device. The method is highly selective, sensitive, rapid and consumes minute amount of reagents. The developed method is free of interference from the degradation products of MEB and from common ingredients present in pharmaceutical formulations. The limit of detection was 0.043 μg/mL, and the limit of quantification was 0.138 μg/mL. The short analysis time per sample (20 s) allowed a large number of analyses to be performed within a very short time. Various samples were analyzed, including two different pharmaceutical formulations and a uniformity of content analysis for 20 tablets from a known batch and two biological samples at different concentrations. In addition, the method was compared with a validated high-performance liquid chromatography (HPLC) method and the results clearly indicated the suitability of the developed method for routine analyses. A new mechanism for the tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3 2+)- peroxodisulfate (S2O8 2-) chemiluminescence (CL) system has also been proposed. The mechanism is based on photoinduced oxidation of Ru(bpy)3 2+ to Ru(bpy)3 3+ via the formation of Ru(bpy)3 2+* upon irradiation with visible light. S2O8 2- then oxidizes Ru(bpy)3 2+* to Ru(bpy)3 3+ and the analyte subsequently reduces the resultant Ru(bpy)3 3+ to Ru(bpy)3 2+*, which then produces the CL signal.

KW - chemiluminscence

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KW - microfluidics

KW - photoinduced oxidation

KW - tris(2,2-bipyridyl)ruthenium(II)

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