Pathogenicity confirmation of Ganoderma disease of coconut using early diagnosis technique

M. Karthikeyan, K. Radhika, R. Bhaskaran, S. Mathiyazhagan, R. Samiyappan, R. Velazhahan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The pathogenicity and diagnostic methods were standardized for Ganoderma disease of coconut. The pathogenicity of Ganoderma lucidum isolated from coconut was tested using six types of inoculation techniques. Two diagnostic methods, viz. indirect enzyme-linked immunosorbent assay (ELISA) and polymerase chain reactions (PCR) were applied for the confirmation of pathogenicity in coconut seedlings. Polyclonal antibodies (PAbs) were raised against mycelial, basidiocarp and specific proteins of Ganoderma and used for detection of Ganoderma in inoculated seedlings through indirect ELISA technique. All the three PAbs could detect Ganoderma in diseased coconut root tissues in early stage of the disease before symptom expression by indirect - ELISA at the antiserum dilution of 1: 1000 for mycelial protein, 1: 700 for Ganoderma specific protein and 1: 3000 for basidiocarp protein. Low cross-reactions were observed with saprophytic fungi occurring in coconut roots and also with other basidiomycetous fungi. In PCR, primers Gan1 and Gan2 generated from internal transcribed spacer region of rDNA were used the detection that produced a product of 167-bp size in Ganoderma infected plants. In the present investigation, spawn inoculum responded earlier within 8 weeks compared with other methods of inoculation as expressed by OD value in ELISA test. This was also confirmed by PCR technique. The combination of these two diagnostic methods for detection of Ganoderma infection was highly reliable, rapid and sensitive.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalJournal of Phytopathology
Volume155
Issue number5
DOIs
Publication statusPublished - May 2007

Fingerprint

Ganoderma
Cocos
early diagnosis
coconuts
Virulence
Early Diagnosis
pathogenicity
Fungal Fruiting Bodies
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
diagnostic techniques
basidiomata
polymerase chain reaction
polyclonal antibodies
Seedlings
Polymerase Chain Reaction
methodology
Proteins
Fungi
proteins

Keywords

  • Coconut
  • ELISA
  • Ganoderma
  • Pathogenicity
  • PCR
  • Polyclonal antibodies

ASJC Scopus subject areas

  • Plant Science

Cite this

Pathogenicity confirmation of Ganoderma disease of coconut using early diagnosis technique. / Karthikeyan, M.; Radhika, K.; Bhaskaran, R.; Mathiyazhagan, S.; Samiyappan, R.; Velazhahan, R.

In: Journal of Phytopathology, Vol. 155, No. 5, 05.2007, p. 296-304.

Research output: Contribution to journalArticle

Karthikeyan, M. ; Radhika, K. ; Bhaskaran, R. ; Mathiyazhagan, S. ; Samiyappan, R. ; Velazhahan, R. / Pathogenicity confirmation of Ganoderma disease of coconut using early diagnosis technique. In: Journal of Phytopathology. 2007 ; Vol. 155, No. 5. pp. 296-304.
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