Parallel microdevice for high throughput analysis of levofloxacin using tris (2,2'-bipyridyl) ruthenium (II) and peroxydisulfate chemiluminescence system

Haider A J Al Lawati, Eiman Al Gharibi, Salma M Z Al Kindy, Fakhr Eldin O Suliman, Ali M. Al-Lawati

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A parallel microdevice has been developed for high throughput analysis using microfluidics. The detection method is based on a chemiluminescence (CL) system based on the oxidation of tris (2,2'-bipyridyl) Ru(II) [Ru (bipy) 32+] by peroxydisulfate. The device consists of a photoreactor chip and two detection chips. The sample throughput can reach up to 720 runs/h with the total reagent consumption of only 2.4 mL. The parallel microdevice was evaluated using levofloxacin (LEVO) in pharmaceutical preparations. The various factors that affect the CL signal were optimized, and the LOD was found to be 30 and 27 μg/L for the two detectors, respectively (S/N = 3), while RSD was 1.1% (n = 15) for 1.0 mg/L LEVO. Two tablet samples that contain LEVO as an active ingredient were successfully analyzed using the proposed parallel microdevice.

Original languageEnglish
Pages (from-to)1056-1060
Number of pages5
JournalJournal of AOAC International
Volume97
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

ruthenium
Levofloxacin
Chemiluminescence
chemiluminescence
detection method
Luminescence
drug
Throughput
oxidation
Microfluidic Analytical Techniques
2,2'-Dipyridyl
active ingredients
Microfluidics
Tablets
detectors
Detectors
sampling
drugs
Equipment and Supplies
Oxidation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Agronomy and Crop Science
  • Food Science
  • Environmental Chemistry
  • Pharmacology

Cite this

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title = "Parallel microdevice for high throughput analysis of levofloxacin using tris (2,2'-bipyridyl) ruthenium (II) and peroxydisulfate chemiluminescence system",
abstract = "A parallel microdevice has been developed for high throughput analysis using microfluidics. The detection method is based on a chemiluminescence (CL) system based on the oxidation of tris (2,2'-bipyridyl) Ru(II) [Ru (bipy) 32+] by peroxydisulfate. The device consists of a photoreactor chip and two detection chips. The sample throughput can reach up to 720 runs/h with the total reagent consumption of only 2.4 mL. The parallel microdevice was evaluated using levofloxacin (LEVO) in pharmaceutical preparations. The various factors that affect the CL signal were optimized, and the LOD was found to be 30 and 27 μg/L for the two detectors, respectively (S/N = 3), while RSD was 1.1{\%} (n = 15) for 1.0 mg/L LEVO. Two tablet samples that contain LEVO as an active ingredient were successfully analyzed using the proposed parallel microdevice.",
author = "{Al Lawati}, {Haider A J} and {Al Gharibi}, Eiman and {Al Kindy}, {Salma M Z} and Suliman, {Fakhr Eldin O} and Al-Lawati, {Ali M.}",
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AU - Al Lawati, Haider A J

AU - Al Gharibi, Eiman

AU - Al Kindy, Salma M Z

AU - Suliman, Fakhr Eldin O

AU - Al-Lawati, Ali M.

PY - 2014

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N2 - A parallel microdevice has been developed for high throughput analysis using microfluidics. The detection method is based on a chemiluminescence (CL) system based on the oxidation of tris (2,2'-bipyridyl) Ru(II) [Ru (bipy) 32+] by peroxydisulfate. The device consists of a photoreactor chip and two detection chips. The sample throughput can reach up to 720 runs/h with the total reagent consumption of only 2.4 mL. The parallel microdevice was evaluated using levofloxacin (LEVO) in pharmaceutical preparations. The various factors that affect the CL signal were optimized, and the LOD was found to be 30 and 27 μg/L for the two detectors, respectively (S/N = 3), while RSD was 1.1% (n = 15) for 1.0 mg/L LEVO. Two tablet samples that contain LEVO as an active ingredient were successfully analyzed using the proposed parallel microdevice.

AB - A parallel microdevice has been developed for high throughput analysis using microfluidics. The detection method is based on a chemiluminescence (CL) system based on the oxidation of tris (2,2'-bipyridyl) Ru(II) [Ru (bipy) 32+] by peroxydisulfate. The device consists of a photoreactor chip and two detection chips. The sample throughput can reach up to 720 runs/h with the total reagent consumption of only 2.4 mL. The parallel microdevice was evaluated using levofloxacin (LEVO) in pharmaceutical preparations. The various factors that affect the CL signal were optimized, and the LOD was found to be 30 and 27 μg/L for the two detectors, respectively (S/N = 3), while RSD was 1.1% (n = 15) for 1.0 mg/L LEVO. Two tablet samples that contain LEVO as an active ingredient were successfully analyzed using the proposed parallel microdevice.

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