Abstract
Background/Aims: Epidemiologically, diabetics are more prone to the adverse health effects of particulate air pollution than healthy individuals. We recently demonstrated an increased cardiovascular and respiratory susceptibility to diesel exhaust particles (DEP) in mice with type 1 diabetes. However, the pancreatic effects of DEP in healthy and diabetic mice are unknown. Methods: Presently, we evaluated the pancreatic impact of DEP in healthy mice, and mice with streptozotocin-induced type 1 diabetes. Four weeks following induction of diabetes, mice were intratracheally instilled (i.t.) with either DEP (0.4 mg/kg) or saline, and several histological and biochemical endpoints were measured 24 h thereafter. Results: Neither the histology nor the stain for apoptosis in the pancreatic islets and exocrine glands were affected by DEP. In diabetic mice exposed to saline, the islet cells showed cellular vacuolation and apoptotic islet cells (71.6 ± 2.6%). In diabetic mice exposed to DEP, a more marked decrease in the size and number of islet cells with cellular vacuolation along with a significant increase of apoptotic islet cells (79.1 ± 1.7 %, P
| Original language | English |
|---|---|
| Pages (from-to) | 413-422 |
| Number of pages | 10 |
| Journal | Cellular Physiology and Biochemistry |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Diesel exhaust particles
- Mice
- Oxidative stress
- Pancreas
- Particulate air pollution
- Streptozotocin
- Type I diabetes
ASJC Scopus subject areas
- Physiology
Cite this
Pancreatic effects of diesel exhaust particles in mice with type 1 diabetes mellitus. / Nemmar, Abderrahim; Al-Salam, Suhail; Beegam, Sumaya; Yuvaraju, Priya; Yasin, Javed; Ali, Badreldin H.
In: Cellular Physiology and Biochemistry, Vol. 33, No. 2, 2014, p. 413-422.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pancreatic effects of diesel exhaust particles in mice with type 1 diabetes mellitus
AU - Nemmar, Abderrahim
AU - Al-Salam, Suhail
AU - Beegam, Sumaya
AU - Yuvaraju, Priya
AU - Yasin, Javed
AU - Ali, Badreldin H.
PY - 2014
Y1 - 2014
N2 - Background/Aims: Epidemiologically, diabetics are more prone to the adverse health effects of particulate air pollution than healthy individuals. We recently demonstrated an increased cardiovascular and respiratory susceptibility to diesel exhaust particles (DEP) in mice with type 1 diabetes. However, the pancreatic effects of DEP in healthy and diabetic mice are unknown. Methods: Presently, we evaluated the pancreatic impact of DEP in healthy mice, and mice with streptozotocin-induced type 1 diabetes. Four weeks following induction of diabetes, mice were intratracheally instilled (i.t.) with either DEP (0.4 mg/kg) or saline, and several histological and biochemical endpoints were measured 24 h thereafter. Results: Neither the histology nor the stain for apoptosis in the pancreatic islets and exocrine glands were affected by DEP. In diabetic mice exposed to saline, the islet cells showed cellular vacuolation and apoptotic islet cells (71.6 ± 2.6%). In diabetic mice exposed to DEP, a more marked decrease in the size and number of islet cells with cellular vacuolation along with a significant increase of apoptotic islet cells (79.1 ± 1.7 %, P
AB - Background/Aims: Epidemiologically, diabetics are more prone to the adverse health effects of particulate air pollution than healthy individuals. We recently demonstrated an increased cardiovascular and respiratory susceptibility to diesel exhaust particles (DEP) in mice with type 1 diabetes. However, the pancreatic effects of DEP in healthy and diabetic mice are unknown. Methods: Presently, we evaluated the pancreatic impact of DEP in healthy mice, and mice with streptozotocin-induced type 1 diabetes. Four weeks following induction of diabetes, mice were intratracheally instilled (i.t.) with either DEP (0.4 mg/kg) or saline, and several histological and biochemical endpoints were measured 24 h thereafter. Results: Neither the histology nor the stain for apoptosis in the pancreatic islets and exocrine glands were affected by DEP. In diabetic mice exposed to saline, the islet cells showed cellular vacuolation and apoptotic islet cells (71.6 ± 2.6%). In diabetic mice exposed to DEP, a more marked decrease in the size and number of islet cells with cellular vacuolation along with a significant increase of apoptotic islet cells (79.1 ± 1.7 %, P
KW - Diesel exhaust particles
KW - Mice
KW - Oxidative stress
KW - Pancreas
KW - Particulate air pollution
KW - Streptozotocin
KW - Type I diabetes
UR - http://www.scopus.com/inward/record.url?scp=84895600193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895600193&partnerID=8YFLogxK
U2 - 10.1159/000356680
DO - 10.1159/000356680
M3 - Article
VL - 33
SP - 413
EP - 422
JO - Cellular Physiology and Biochemistry
JF - Cellular Physiology and Biochemistry
SN - 1015-8987
IS - 2
ER -